Purification of specific chromatin loci for proteomic analysis

Stephanie D. Byrum; Sean D. Taverna; Alan J. Tackett

doi:10.1007/978-1-4939-1680-1_8

Purification of specific chromatin loci for proteomic analysis

Stephanie D. Byrum, Sean D. Taverna, Alan J. Tackett

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Purification of small, native chromatin regions for proteomic identification of specifically bound proteins and histone posttranslational modifications is a powerful approach for studying mechanisms of chromosome metabolism. Here we detail a C hromatin A ffinity P urification with M ass S pectrometry (ChAP-MS) approach for affinity purification of 1 kb regions of chromatin for targeted proteomic analysis. This approach utilizes quantitative, high resolution mass spectrometry to categorize proteins and histone posttranslational modifications co-enriched with the given chromatin region as either “specific” to the targeted chromatin or “nonspecific” contamination. In this way, the ChAP-MS approach can help define and redefine mechanisms of chromatin-templated activities.

Original language	English (US)
Pages (from-to)	83-92
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1228
DOIs	https://doi.org/10.1007/978-1-4939-1680-1_8
State	Published - 2015

Keywords

Chromatin
Epigenetics
Histone
Posttranslational modification
Proteomics
Purification

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-1680-1_8

Cite this

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title = "Purification of specific chromatin loci for proteomic analysis",

abstract = "Purification of small, native chromatin regions for proteomic identification of specifically bound proteins and histone posttranslational modifications is a powerful approach for studying mechanisms of chromosome metabolism. Here we detail a C hromatin A ffinity P urification with M ass S pectrometry (ChAP-MS) approach for affinity purification of 1 kb regions of chromatin for targeted proteomic analysis. This approach utilizes quantitative, high resolution mass spectrometry to categorize proteins and histone posttranslational modifications co-enriched with the given chromatin region as either “specific” to the targeted chromatin or “nonspecific” contamination. In this way, the ChAP-MS approach can help define and redefine mechanisms of chromatin-templated activities.",

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T1 - Purification of specific chromatin loci for proteomic analysis

AU - Byrum, Stephanie D.

AU - Taverna, Sean D.

AU - Tackett, Alan J.

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PY - 2015

Y1 - 2015

N2 - Purification of small, native chromatin regions for proteomic identification of specifically bound proteins and histone posttranslational modifications is a powerful approach for studying mechanisms of chromosome metabolism. Here we detail a C hromatin A ffinity P urification with M ass S pectrometry (ChAP-MS) approach for affinity purification of 1 kb regions of chromatin for targeted proteomic analysis. This approach utilizes quantitative, high resolution mass spectrometry to categorize proteins and histone posttranslational modifications co-enriched with the given chromatin region as either “specific” to the targeted chromatin or “nonspecific” contamination. In this way, the ChAP-MS approach can help define and redefine mechanisms of chromatin-templated activities.

AB - Purification of small, native chromatin regions for proteomic identification of specifically bound proteins and histone posttranslational modifications is a powerful approach for studying mechanisms of chromosome metabolism. Here we detail a C hromatin A ffinity P urification with M ass S pectrometry (ChAP-MS) approach for affinity purification of 1 kb regions of chromatin for targeted proteomic analysis. This approach utilizes quantitative, high resolution mass spectrometry to categorize proteins and histone posttranslational modifications co-enriched with the given chromatin region as either “specific” to the targeted chromatin or “nonspecific” contamination. In this way, the ChAP-MS approach can help define and redefine mechanisms of chromatin-templated activities.

KW - Chromatin

KW - Epigenetics

KW - Histone

KW - Posttranslational modification

KW - Proteomics

KW - Purification

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Purification of specific chromatin loci for proteomic analysis

Abstract

Keywords

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