Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining

J. S. Zigler, M. Mochizuki, T. Kuwabara, I. Gery

Research output: Contribution to journalArticle

Abstract

This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 columm. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.

Original languageEnglish (US)
Pages (from-to)977-980
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume25
Issue number8
StatePublished - Jan 1 1984

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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