Purification of human seminal acrosin inhibitor and its kinetics

Aditi Chatterjee, Sagarika Kanjilal, Asok K. Bhattacharyya

Research output: Contribution to journalArticle

Abstract

A low molecular mass, naturally occurring acrosin inhibitor has been identified and purified (490.7-fold) from human semen, and kinetic studies have been performed on the association characteristics as well as for the determination of affinity constants (K(i) values). The results show that K(i) value (3.34 x 10-2) of the inhibitor towards human acrosin is almost three times lower than that of pancreatic trypsin, indicating a much higher specificity and inhibitory property for acrosin. The purified human seminal acrosin inhibitor has a molecular mass of 5.5 kDa and shows a single band using 10-20% gradient SDS PAGE. The work is of great significance for the development of more specific, nontoxic and irreversible inhibitors for human acrosin.

Original languageEnglish (US)
Pages (from-to)323-328
Number of pages6
JournalJournal of Biosciences
Volume24
Issue number3
DOIs
StatePublished - Sep 1999

Keywords

  • Human acrosin
  • Kinetics
  • Seminal inhibitor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Chatterjee, A., Kanjilal, S., & Bhattacharyya, A. K. (1999). Purification of human seminal acrosin inhibitor and its kinetics. Journal of Biosciences, 24(3), 323-328. https://doi.org/10.1007/BF02941246