Purification of Giardia muris trophozoites by using nylon fiber columns

J. S. Andrews, J. J. Ellner, D. P. Stevens

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 106 viable purified trophozoites per infected mouse.

Original languageEnglish (US)
Pages (from-to)12-15
Number of pages4
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume29
Issue number1
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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