A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 106 viable purified trophozoites per infected mouse.
|Original language||English (US)|
|Number of pages||4|
|Journal||American Journal of Tropical Medicine and Hygiene|
|State||Published - 1980|
ASJC Scopus subject areas
- Infectious Diseases