Purification of a glycoprotein bearing a tumor transplantation antigen specific for Friend, Moloney, and Rauscher MuLV-induced tumors

M. J. Rogers, G. Galetto, V. J. Hearing, D. F. Siwarski, L. W. Law

Research output: Contribution to journalArticlepeer-review

Abstract

A 175,000 dalton glycoprotein bearing a tumor associated transplantation antigen (TATA) has been isolated from a Rauscher-MuLV-induced leukemia (RBL-5). The glycoprotein has the properties expected for a Friend, Moloney, or Rauscher-MuLV-induced TATA: 1) It is synthesized by RBL-5 cells and is expressed on the cell surface. 2) When inoculated into mice, it provides complete protection against challenge with RBL-5 cells at doses of 1 μg or less. 3) The same protein, isolated from a Moloney-MuLV-induced leukemia, also protects mice against challenge with RBL-5. A similar glycoprotein is expressed on other tumor cells and on normal cells that do not express a TATA cross-reactive with RBL-5. Therefore, the immunogenicity of this molecule cannot be principally accounted for by either its unique or elevated expression on RBL-5 cells. A model is presented that accounts for these properties of the RBL-5 TATA. In addition, two other features of the RBL-5 TATA are discussed; an apparent abrogation of immunity seen only with certain doses of antigen, and a loss of the inherent immunogenicity of gp175 during purification.

Original languageEnglish (US)
Pages (from-to)3211-3217
Number of pages7
JournalJournal of Immunology
Volume132
Issue number6
StatePublished - Jul 19 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Purification of a glycoprotein bearing a tumor transplantation antigen specific for Friend, Moloney, and Rauscher MuLV-induced tumors'. Together they form a unique fingerprint.

Cite this