Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria

Laura N. Rusché, Jorge Cruz-Reyes, Kenneth J. Piller, Barbara Sollner-Webb

Research output: Contribution to journalArticle

Abstract

Kinetoplastid mitochondrial RNA editing, the insertion and deletion of U residues, is catalyzed by sequential cleavage, U addition or removal, and ligation reactions and is directed by complementary guide RNAs. We have purified a ~20S enzymatic complex from Trypanosoma brucei mitochondria that catalyzes a complete editing reaction in vitro. This complex possesses all four activities predicted to catalyze RNA editing: gRNA-directed endonuclease, terminal uridylyl transferase, 3' U-specific exonuclease, and RNA ligase. However, it does not contain other putative editing complex components: gRNA-independent endonuclease, RNA helicase, endogenous gRNAs or pre-mRNAs, or a 25 kDa gRNA-binding protein. The complex is composed of eight major polypeptides, three of which represent RNA ligase. These findings identify polypeptides representing catalytic editing factors, reveal the nature of this ~20S editing complex, and suggest a new model of editosome assembly.

Original languageEnglish (US)
Pages (from-to)4069-4081
Number of pages13
JournalEMBO Journal
Volume16
Issue number13
DOIs
StatePublished - Jul 1 1997

Keywords

  • Editing complex
  • RNA editing
  • RNA lipase
  • Trypanosoma brucei
  • Trypanosome

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria'. Together they form a unique fingerprint.

  • Cite this

    Rusché, L. N., Cruz-Reyes, J., Piller, K. J., & Sollner-Webb, B. (1997). Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria. EMBO Journal, 16(13), 4069-4081. https://doi.org/10.1093/emboj/16.13.4069