Purification, characterization, and chemical modification of neurotoxic peptide from daboia russelii snake venom of india

Madhukumar Venkatesh, Nijaguna Prasad, Tej Sing, Veerabasappa Gowda

Research output: Contribution to journalArticlepeer-review

Abstract

Comprehensive knowledge of venom composition is very important for effective management of snake envenomation and antivenom preparation. Daboia russelii venom from the eastern region of India is the most neurotoxic among the four venom samples investigated. From the eastern D. russelii venom sample, neurotoxic peptide has been purified by combined method of ion exchange gel permeation chromatography and reversed phase high performance liquid chromatography. Molecular weight of Daboia neurotoxin III (DNTx-III) found to be 6,849 Da (as measured on matrix-assisted laser desorption/ionisation-time of flight mass spectrometer), and N-terminal amino acid sequences is I K C F I T P D U T S Q A. Approximate LD50 dosage was 0.24 mg/kg body weight. It produced concentration- and time-dependent inhibition of indirectly stimulated twitches of Rana hexadactyla sciatic nerve gastrocnemius muscle preparations. Chemical modification of DNTx-III tryptophan residue(s) reduced the twitch height inhibition property of toxin, signifying the importance of tryptophan residues for the neurotoxic function. This type of neurotoxic peptide is unique to east Indian regional D. russelii venom.

Original languageEnglish (US)
Pages (from-to)295-304
Number of pages10
JournalJournal of Biochemical and Molecular Toxicology
Volume27
Issue number6
DOIs
StatePublished - Jun 1 2013

Keywords

  • Antivenom
  • Daboia russelii
  • Neurotoxin
  • Russell's viper
  • Snake venom

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Purification, characterization, and chemical modification of neurotoxic peptide from daboia russelii snake venom of india'. Together they form a unique fingerprint.

Cite this