Purification and partial amino acid sequence analysis of human erythrocyte acetylcholinesterase

Vijay Chhajlani, Dwight Derr, Betty Earles, Eli Schmell, Thomas August

Research output: Contribution to journalArticle

Abstract

A single step immunoaffinity purification procedure for human erythrocyte acetylcholinesterase is described which permitted the isolation of milligram quantities of enzyme from 10 U of erythrocytes, with 113 000-fold purification and a yield of about 22%. In SDS-PAGE analysis, the enzyme corresponds to a disulfide linked dimer of 140 kDa which is converted to a 70 kDa monomer upon disulfide reduction. The tryptic peptides generated from purified enzyme were separated by reverse-phase HPLC. Five of these peptides were analysed to determine the amino acid sequences. The obtained sequences showed no homology to the already known amino acid sequences for human serum and brain butyryl-cholinesterase and Torpedo californica acetylcholinesterase.

Original languageEnglish (US)
Pages (from-to)279-282
Number of pages4
JournalFEBS Letters
Volume247
Issue number2
DOIs
StatePublished - Apr 24 1989

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Protein Sequence Analysis
Acetylcholinesterase
Purification
Erythrocytes
Amino Acids
Disulfides
Amino Acid Sequence
Enzymes
Torpedo
Peptides
Cholinesterases
Dimers
Polyacrylamide Gel Electrophoresis
Brain
Monomers
High Pressure Liquid Chromatography
Serum

Keywords

  • Acetylcholinesterase
  • Amino acid sequence
  • Immunoaffinity chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Purification and partial amino acid sequence analysis of human erythrocyte acetylcholinesterase. / Chhajlani, Vijay; Derr, Dwight; Earles, Betty; Schmell, Eli; August, Thomas.

In: FEBS Letters, Vol. 247, No. 2, 24.04.1989, p. 279-282.

Research output: Contribution to journalArticle

Chhajlani, Vijay ; Derr, Dwight ; Earles, Betty ; Schmell, Eli ; August, Thomas. / Purification and partial amino acid sequence analysis of human erythrocyte acetylcholinesterase. In: FEBS Letters. 1989 ; Vol. 247, No. 2. pp. 279-282.
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