Purification and expansion of human hematopoietic stem/progenitor cells

C. I. Civin, Donald Small

Research output: Contribution to journalArticle

Abstract

CD34 monoclonal antibodies bind selectively to the most immature 1.5% of low-density human bone marrow mononuclear cells, including terminal deoxynucleotidyl transferase-positive lymphoid precursor cells, all types of in vitro assayed hematopoietic progenitor cells, and lymphohematopoietic stem cells capable of reconstituting myeloablated humans in clinical transplantation. Positive selection of highly enriched CD34+ stem and progenitor cells is widely used in research and is now being investigated in many applications of autologous and allogeneic clinical transplantation. More highly purified stem cells are also desired in research and may have clinical use. Immunoaffinity isolation of CD34+ cell subsets using antibodies against CD38 permits 10-100-fold further purification of stem cells. It will be valuable to be able to expand stem and progenitor cells from small marrow and blood samples. We are now identifying the genes expressed in stem and progenitor cells to eventually allow the control of stem and progenitor cell survival, proliferation, and differentiation.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalAnnals of the New York Academy of Sciences
Volume770
DOIs
StatePublished - 1995

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Hematopoietic Stem Cells
Stem cells
Purification
Stem Cells
DNA Nucleotidylexotransferase
Cell proliferation
Bone
Blood
Genes
Monoclonal Antibodies
Antibodies
Cells
Cell Separation
Homologous Transplantation
Research
Bone Marrow Cells
Cell Differentiation
Cell Survival
Transplantation
Bone Marrow

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Purification and expansion of human hematopoietic stem/progenitor cells. / Civin, C. I.; Small, Donald.

In: Annals of the New York Academy of Sciences, Vol. 770, 1995, p. 91-98.

Research output: Contribution to journalArticle

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