Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

J. R. Scocca; G. H. Thomas; C. Miller; L. Reynolds

doi:10.1007/BF01799486

Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

J. R. Scocca, G. H. Thomas, C. Miller, L. Reynolds

Research output: Contribution to journal › Article › peer-review

Abstract

Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [³H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.

Original language	English (US)
Pages (from-to)	33-47
Number of pages	15
Journal	Journal of Inherited Metabolic Disease
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF01799486
State	Published - Mar 1987

ASJC Scopus subject areas

Genetics
Genetics(clinical)

Access to Document

10.1007/BF01799486

Cite this

@article{c706b35b0d7849ca86e73ed60eef24c3,

title = "Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis",

abstract = "Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [3H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.",

author = "Scocca, {J. R.} and Thomas, {G. H.} and C. Miller and L. Reynolds",

year = "1987",

month = mar,

doi = "10.1007/BF01799486",

language = "English (US)",

volume = "10",

pages = "33--47",

journal = "Journal of Inherited Metabolic Disease",

issn = "0141-8955",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

AU - Scocca, J. R.

AU - Thomas, G. H.

AU - Miller, C.

AU - Reynolds, L.

PY - 1987/3

Y1 - 1987/3

N2 - Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [3H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.

AB - Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [3H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.

UR - http://www.scopus.com/inward/record.url?scp=0023093540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023093540&partnerID=8YFLogxK

U2 - 10.1007/BF01799486

DO - 10.1007/BF01799486

M3 - Article

C2 - 3106715

AN - SCOPUS:0023093540

SN - 0141-8955

VL - 10

SP - 33

EP - 47

JO - Journal of Inherited Metabolic Disease

JF - Journal of Inherited Metabolic Disease

IS - 1

ER -

Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this