Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

J. R. Scocca, G. H. Thomas, C. Miller, L. Reynolds

Research output: Contribution to journalArticle

Abstract

Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [3H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.

Original languageEnglish (US)
Pages (from-to)33-47
Number of pages15
JournalJournal of Inherited Metabolic Disease
Volume10
Issue number1
DOIs
StatePublished - Mar 1987

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Amino Sugars
Asparagine
Neuraminidase
N-Acetylneuraminic Acid
Gel Chromatography
Anions
Glycoproteins
Fibroblasts
Carbohydrates
Skin
sialooligosaccharides
N-acetylmannosamine
Neuraminidase 1 deficiency

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Endocrinology

Cite this

Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis. / Scocca, J. R.; Thomas, G. H.; Miller, C.; Reynolds, L.

In: Journal of Inherited Metabolic Disease, Vol. 10, No. 1, 03.1987, p. 33-47.

Research output: Contribution to journalArticle

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