Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

J. R. Scocca; G. H. Thomas; C. Miller; L. Reynolds

doi:10.1007/BF01799486

Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

J. R. Scocca, G. H. Thomas, C. Miller, L. Reynolds

Research output: Contribution to journal › Article › peer-review

Abstract

Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [³H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.

Original language	English (US)
Pages (from-to)	33-47
Number of pages	15
Journal	Journal of Inherited Metabolic Disease
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF01799486
State	Published - Mar 1987
Externally published	Yes

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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abstract = "Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [3H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.",

author = "Scocca, {J. R.} and Thomas, {G. H.} and C. Miller and L. Reynolds",

year = "1987",

month = mar,

doi = "10.1007/BF01799486",

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AU - Scocca, J. R.

AU - Thomas, G. H.

AU - Miller, C.

AU - Reynolds, L.

PY - 1987/3

Y1 - 1987/3

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AB - Sialyl conjugates which accumulate in sialidase deficient fibroblasts were made radioactive in the sialic acid moiety by culturing the cells in the presence of [3H]N-acetylmannosamine. The accumulated radioactive compounds were fractionated by anion exchange chromatography and gel filtration. At least nine chromatographically distinct sialyl conjugates were obtained. Neutral and amino sugar analysis of these compounds indicated that all were sialyloligosaccharides of the type which could be derived from glycoproteins containing complex, N-asparagine-linked carbohydrate groups.

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Purification and characterization of sialic acid containing materials accumulated in cultured skin fibroblasts from a patient with type II sialidosis

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