Purification And Characterization Of A Human-Specific Esterase From Urine

G. D. Therrien, N. R. Rose, W. R. Bartholomew

Research output: Contribution to journalArticle

Abstract

A human-specific esterase was Isolated from urine and partially characterized by ammonium sulfate precipitation, starch block electrophoresis, and gel filtration. Its molecular weight was estimated to be 136,000 by polyacrylamide gel electrophoresis in 0.17. SDS. A diffusion coefficient of 8.6 X 10 7was determined by the L-plate method. Inmunologic identity was shown between the urinary esterase and a human tissue esterase.

Original languageEnglish (US)
Pages (from-to)259-268
Number of pages10
JournalPreparative Biochemistry
Volume1
Issue number1
DOIs
StatePublished - Jan 1 1971

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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