Purification And Characterization Of A Human-Specific Esterase From Urine

G. D. Therrien; N. R. Rose; W. R. Bartholomew

doi:10.1080/00327487108081944

Purification And Characterization Of A Human-Specific Esterase From Urine

G. D. Therrien, N. R. Rose, W. R. Bartholomew

Research output: Contribution to journal › Article › peer-review

Abstract

A human-specific esterase was Isolated from urine and partially characterized by ammonium sulfate precipitation, starch block electrophoresis, and gel filtration. Its molecular weight was estimated to be 136,000 by polyacrylamide gel electrophoresis in 0.17. SDS. A diffusion coefficient of 8.6 X 10 ⁷was determined by the L-plate method. Inmunologic identity was shown between the urinary esterase and a human tissue esterase.

Original language	English (US)
Pages (from-to)	259-268
Number of pages	10
Journal	Preparative Biochemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1080/00327487108081944
State	Published - Jan 1 1971
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Genetics

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10.1080/00327487108081944

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abstract = "A human-specific esterase was Isolated from urine and partially characterized by ammonium sulfate precipitation, starch block electrophoresis, and gel filtration. Its molecular weight was estimated to be 136,000 by polyacrylamide gel electrophoresis in 0.17. SDS. A diffusion coefficient of 8.6 X 10 7was determined by the L-plate method. Inmunologic identity was shown between the urinary esterase and a human tissue esterase.",

author = "Therrien, {G. D.} and Rose, {N. R.} and Bartholomew, {W. R.}",

note = "Funding Information: by PRS research grant CA-05203 from the National Cancer Institute and",

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Purification And Characterization Of A Human-Specific Esterase From Urine

Abstract

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