Pulmonary T cell repertoire in patients with graft-versus-host disease following blood and marrow transplantation

Vladimir V. Yurovsky, Michele H. Cottler-Fox, Sergei P. Atamas, Carl B. Shanholtz, E. James Britt, Lyle L. Sensenbrenner, Barbara White

Research output: Contribution to journalArticle

Abstract

Pulmonary inflammation is one of the risk factors associated with blood and marrow transplantation (BMT). To determine the potential role of T cells in pulmonary complications after transplantation, we analyzed the T-cell repertoire expressed in bronchoalveolar lavage fluids from eleven patients with graft-versus-host disease following BMT. A reverse transcriptase-polymerase chain reaction was used to amplify rearranged TCR transcripts in unfractionated, CD4+, and CD8+ T cells from bronchoalveolar lavage fluids. The relative expression of TCR variable (V) gene families and the diversity of junctional region lengths associated with different AV and BV gene families were analyzed. Nearly all TCR AV and BV gene families were detected in bronchoalveolar lavage cells from BMT recipients. Oligoclonal patterns of TCR junctional region lengths were observed in unfractionated, CD4+, and CD8+ bronchoalveolar T cells. The oligoclonal expansion of bronchoalveolar T cells in patients was confirmed by DNA sequencing. TCRV gene expression is almost completely restored in the lungs of BMT recipients as early as two weeks after transplantation. Increased oligoclonality among TCR gene families suggests either an incomplete restoration of TCR diversity or an antigen-driven expansion of T cells in the lungs of BMT recipients with graft-versus-host disease, not necessarily related to pulmonary infection. (C) 2001 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalAmerican Journal of Hematology
Volume66
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

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Keywords

  • Graft-versus-host disease
  • Hematopoietic stem cell transplantation
  • Pulmonary inflammation
  • T cell
  • T-cell receptor

ASJC Scopus subject areas

  • Hematology

Cite this

Yurovsky, V. V., Cottler-Fox, M. H., Atamas, S. P., Shanholtz, C. B., James Britt, E., Sensenbrenner, L. L., & White, B. (2001). Pulmonary T cell repertoire in patients with graft-versus-host disease following blood and marrow transplantation. American Journal of Hematology, 66(1), 1-11. https://doi.org/10.1002/1096-8652(200101)66:1<1::AID-AJH1000>3.0.CO;2-R