Pulmonary surfactant metabolism in infants lacking surfactant protein B

Michael F. Beers, Aaron Hamvas, Michael A. Moxley, Linda W. Gonzales, Susan H. Guttentag, Kola O. Solarin, William J. Longmore, Lawrence Nogee, Philip L. Ballard

Research output: Contribution to journalArticle

Abstract

Infants with inherited deficiency of pulmonary surfactant protein (SP) B develop respiratory failure at birth and die without lung transplantation. We examined aspects of surfactant metabolism in lung tissue and lavage fluid acquired at transplantation or postmortem from ten infants born at term with inherited deficiency of SP-B; comparison groups were infants with other forms of chronic lung disease (CLD) and normal infants. In pulse/chase labeling studies with cultured deficient tissue, no immunoprecipitable SP-B was observed and an approximately 6-kD form of SP-C accumulated that was only transiently present in CLD tissue. SP-B messenger RNA (mRNA) was approximately 8% of normal in deficient specimens, and some intact message was observed after, but not before, explant culture. Transcription rates for SP-B, assessed by nuclear run-on assay using probes for sequences both 5' and 3' of the common nonsense mutation (121ins2), were comparable in all lungs examined. The minimal surface tension achieved with lavage surfactant was similarly elevated in both deficient and CLD infants (26-31 mN/m) compared with normal infants (6 mN/m). Both SP-B-deficient and CLD infants had markedly decreased phosphatidylglycerol content of lavage and tissue compared with normal lung, whereas synthetic rates for phospholipids, including phosphatidylglycerol, were normal. We conclude that the mutated SP-B gene is transcribed normally but produces an unstable mRNA and that absence of SP-B protein blocks processing of SP-C. Chronic infant lung disease, of various etiologies, reduces surfactant function and apparently alters phosphatidylglycerol degradation.

Original languageEnglish (US)
Pages (from-to)380-391
Number of pages12
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume22
Issue number3
StatePublished - 2000

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Pulmonary Surfactants
Metabolism
Surface-Active Agents
Pulmonary diseases
Lung Diseases
Phosphatidylglycerols
Chronic Disease
Tissue
Therapeutic Irrigation
Protein C
IgA receptor
Pulmonary Surfactant-Associated Proteins
Lung
Messenger RNA
Surface Tension
Lung Transplantation
Nonsense Codon
Bronchoalveolar Lavage Fluid
Transcription
Respiratory Insufficiency

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Beers, M. F., Hamvas, A., Moxley, M. A., Gonzales, L. W., Guttentag, S. H., Solarin, K. O., ... Ballard, P. L. (2000). Pulmonary surfactant metabolism in infants lacking surfactant protein B. American Journal of Respiratory Cell and Molecular Biology, 22(3), 380-391.

Pulmonary surfactant metabolism in infants lacking surfactant protein B. / Beers, Michael F.; Hamvas, Aaron; Moxley, Michael A.; Gonzales, Linda W.; Guttentag, Susan H.; Solarin, Kola O.; Longmore, William J.; Nogee, Lawrence; Ballard, Philip L.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 22, No. 3, 2000, p. 380-391.

Research output: Contribution to journalArticle

Beers, MF, Hamvas, A, Moxley, MA, Gonzales, LW, Guttentag, SH, Solarin, KO, Longmore, WJ, Nogee, L & Ballard, PL 2000, 'Pulmonary surfactant metabolism in infants lacking surfactant protein B', American Journal of Respiratory Cell and Molecular Biology, vol. 22, no. 3, pp. 380-391.
Beers MF, Hamvas A, Moxley MA, Gonzales LW, Guttentag SH, Solarin KO et al. Pulmonary surfactant metabolism in infants lacking surfactant protein B. American Journal of Respiratory Cell and Molecular Biology. 2000;22(3):380-391.
Beers, Michael F. ; Hamvas, Aaron ; Moxley, Michael A. ; Gonzales, Linda W. ; Guttentag, Susan H. ; Solarin, Kola O. ; Longmore, William J. ; Nogee, Lawrence ; Ballard, Philip L. / Pulmonary surfactant metabolism in infants lacking surfactant protein B. In: American Journal of Respiratory Cell and Molecular Biology. 2000 ; Vol. 22, No. 3. pp. 380-391.
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