TY - JOUR
T1 - Pulmonary metastases from autochthonous 3-methylcholanthrene-lnduced murine tumors
AU - Wexler, Hilda
AU - Rosenberg, Steven A.
N1 - Funding Information:
Sarcomas were induced in 6-to 8-week-old female C57BL/6N mice (Small Animal Section, Veterinary Resources Branch, Division of Research Services, National Institutes of Health, Bethesda, Md.) by the injection of 1 mg MCA (Eastman Kodak Co., Rochester, N.Y.) in 0.1 ml sesame oil (Fisher Scientific Co., Fair Lawn, N.].) into the distal muscles of the right hindlimb. When the tumors were 1.5-2.5 cm in diameter (generally 70-110 days), the hindlimb was amputated at the hip with a cutting cautery. Mice were individually housed, and 50 days later they were killed and autopsied to detect metastatic disease. Suspected metastases were examined histologically. If mice appeared moribund prior to 50 days after amputation, they were killed and autopsied for metastatic disease; this was necessary for about 10% of the mice. Any mouse developing a local recurrence at the site of amputation was immediately killed and autopsied. Local recurrences, when they occurred, generally appeared 3-5 weeks after amputation.
Funding Information:
2 Surgery Branch, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Public Health Service, V.S. Department of Health, Education, and Welfare, Bethesda, Md. 20205.
PY - 1979/12
Y1 - 1979/12
N2 - In three experiments, 87%, 75%, and 85% of female C57BL/6N mice developed pulmonary metastases by 50 days after amputation of legs having autochthonous 3-methylcholan-threne-induced sarcomas. No difference in the incidence of pulmonary metastases was observed when autochthonous tumors of short (49-94 days) and longer (95-119 days) latency periods were compared. These metastasizing autochthonous tumors may provide a useful model for studies of metastases and for the evaluation of cancer therapy in a minimal residual disease setting.
AB - In three experiments, 87%, 75%, and 85% of female C57BL/6N mice developed pulmonary metastases by 50 days after amputation of legs having autochthonous 3-methylcholan-threne-induced sarcomas. No difference in the incidence of pulmonary metastases was observed when autochthonous tumors of short (49-94 days) and longer (95-119 days) latency periods were compared. These metastasizing autochthonous tumors may provide a useful model for studies of metastases and for the evaluation of cancer therapy in a minimal residual disease setting.
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U2 - 10.1093/jnci/63.6.1393
DO - 10.1093/jnci/63.6.1393
M3 - Article
C2 - 292810
AN - SCOPUS:0018599273
SN - 0027-8874
VL - 63
SP - 1393
EP - 1395
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -