PU.1 and an HLH family member contribute to the myeloid-specific transcription of the FcγRIIIA promoter

Rena Feinman, Wei Q. Qiu, Roger N. Pearse, Barbara S. Nikolajczyk, Ranjan Sen, Michael Sheffery, Jeffrey V. Ravetch

Research output: Contribution to journalArticle

Abstract

Expression of the low-affinity Fc receptor for IgG (murine FcγRIIIA) is restricted to cells of myelomonocytic origin. We report here the promoter structure the proximal DNA sequences responsible for transcription of FcγRIIIA in macrophages and the protein factors which interact with these sequences. A 51 bp sequence, termed the myeloid restricted region (MRR), was both necessary and sufficient for conferring cell type-specific expression in macrophages. Reporter constructs containing mutations in this sequence result in the loss of MRR activity upon transfection into the macrophage cell line, RAW264.7. Two cis-acting elements have been identified and are required for full promoter function. These same elements analyzed by EMSA define two binding sites recognized by nuclear factors derived from macrophages. A 3' purine tract (-50 to -39) within the MRR binds the macrophage and B cell-specific factor, PU.1, and a second E box-like element, termed MyE, upstream of the PU.1 box (-88 to -78) binds the HLH factors TFE3 and USF. EMSA studies using RAW cell extracts suggest that both PU.1 and MyE factors may bind simultaneously to the MRR resulting in a ternary complex that is responsible, in part, for the myeloid-specific activity of the FcγRIIIA promoter.

Original languageEnglish (US)
Pages (from-to)3852-3860
Number of pages9
JournalThe EMBO journal
Volume13
Issue number16
StatePublished - 1994
Externally publishedYes

Fingerprint

Macrophages
Transcription
Cells
E-Box Elements
Fc Receptors
DNA sequences
Cell Extracts
Transfection
B-Lymphocytes
Immunoglobulin G
Binding Sites
Cell Line
Mutation
Proteins

Keywords

  • FcγRIIIA promoter
  • HLH
  • Myeloid specificity
  • PU.1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Feinman, R., Qiu, W. Q., Pearse, R. N., Nikolajczyk, B. S., Sen, R., Sheffery, M., & Ravetch, J. V. (1994). PU.1 and an HLH family member contribute to the myeloid-specific transcription of the FcγRIIIA promoter. The EMBO journal, 13(16), 3852-3860.

PU.1 and an HLH family member contribute to the myeloid-specific transcription of the FcγRIIIA promoter. / Feinman, Rena; Qiu, Wei Q.; Pearse, Roger N.; Nikolajczyk, Barbara S.; Sen, Ranjan; Sheffery, Michael; Ravetch, Jeffrey V.

In: The EMBO journal, Vol. 13, No. 16, 1994, p. 3852-3860.

Research output: Contribution to journalArticle

Feinman, R, Qiu, WQ, Pearse, RN, Nikolajczyk, BS, Sen, R, Sheffery, M & Ravetch, JV 1994, 'PU.1 and an HLH family member contribute to the myeloid-specific transcription of the FcγRIIIA promoter', The EMBO journal, vol. 13, no. 16, pp. 3852-3860.
Feinman R, Qiu WQ, Pearse RN, Nikolajczyk BS, Sen R, Sheffery M et al. PU.1 and an HLH family member contribute to the myeloid-specific transcription of the FcγRIIIA promoter. The EMBO journal. 1994;13(16):3852-3860.
Feinman, Rena ; Qiu, Wei Q. ; Pearse, Roger N. ; Nikolajczyk, Barbara S. ; Sen, Ranjan ; Sheffery, Michael ; Ravetch, Jeffrey V. / PU.1 and an HLH family member contribute to the myeloid-specific transcription of the FcγRIIIA promoter. In: The EMBO journal. 1994 ; Vol. 13, No. 16. pp. 3852-3860.
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