Psidin, a conserved protein that regulates protrusion dynamics and cell migration

Ji Hoon Kim, Aeri Cho, Hongyan Yin, Dorothy A. Schafer, Ghassan Mouneimne, Kaylene J. Simpson, Kim Vy Nguyen, Joan S. Brugge, Denise J. Montell

Research output: Contribution to journalArticle

Abstract

Dynamic assembly and disassembly of actin filaments is a major driving force for cell movements. Border cells in the Drosophila ovary provide a simple and genetically tractable model to study the mechanisms regulating cell migration. To identify new genes that regulate cell movement in vivo, we screened lethal mutations on chromosome 3R for defects in border cell migration and identified two alleles of the gene psidin (psid). In vitro, purified Psid protein bound F-actin and inhibited the interaction of tropomyosin with F-actin. In vivo, psid mutations exhibited genetic interactions with the genes encoding tropomyosin and cofilin. Border cells overexpressing Psid together with GFP-actin exhibited altered protrusion/retraction dynamics. Psid knockdown in cultured S2 cells reduced, and Psid overexpression enhanced, lamellipodial dynamics. Knockdown of the human homolog of Psid reduced the speed and directionality of migration in wounded MCF10A breast epithelial monolayers, whereas overexpression of the protein increased migration speed and altered protrusion dynamics in EGF-stimulated cells. These results indicate that Psid is an actin regulatory protein that plays a conserved role in protrusion dynamics and cell migration.

Original languageEnglish (US)
Pages (from-to)730-741
Number of pages12
JournalGenes & development
Volume25
Issue number7
DOIs
StatePublished - Apr 2011

Fingerprint

Cell Movement
Actins
Tropomyosin
Proteins
Actin Depolymerizing Factors
Genes
Mutation
Actin Cytoskeleton
Epidermal Growth Factor
Drosophila
Ovary
Cultured Cells
Breast
Chromosomes
Alleles

Keywords

  • Actin
  • Cell migration
  • Drosophila
  • Genetics
  • Protrusion
  • Tropomyosin

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Kim, J. H., Cho, A., Yin, H., Schafer, D. A., Mouneimne, G., Simpson, K. J., ... Montell, D. J. (2011). Psidin, a conserved protein that regulates protrusion dynamics and cell migration. Genes & development, 25(7), 730-741. https://doi.org/10.1101/gad.2028611

Psidin, a conserved protein that regulates protrusion dynamics and cell migration. / Kim, Ji Hoon; Cho, Aeri; Yin, Hongyan; Schafer, Dorothy A.; Mouneimne, Ghassan; Simpson, Kaylene J.; Nguyen, Kim Vy; Brugge, Joan S.; Montell, Denise J.

In: Genes & development, Vol. 25, No. 7, 04.2011, p. 730-741.

Research output: Contribution to journalArticle

Kim, JH, Cho, A, Yin, H, Schafer, DA, Mouneimne, G, Simpson, KJ, Nguyen, KV, Brugge, JS & Montell, DJ 2011, 'Psidin, a conserved protein that regulates protrusion dynamics and cell migration', Genes & development, vol. 25, no. 7, pp. 730-741. https://doi.org/10.1101/gad.2028611
Kim, Ji Hoon ; Cho, Aeri ; Yin, Hongyan ; Schafer, Dorothy A. ; Mouneimne, Ghassan ; Simpson, Kaylene J. ; Nguyen, Kim Vy ; Brugge, Joan S. ; Montell, Denise J. / Psidin, a conserved protein that regulates protrusion dynamics and cell migration. In: Genes & development. 2011 ; Vol. 25, No. 7. pp. 730-741.
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