Pseudo cross-link of human hemoglobin with mono-(3,5-dibromosalicyl)fumarate

E. Bucci, A. Razynska, B. Urbaitis, Clara Fronticelli

Research output: Contribution to journalArticle

Abstract

The reaction of human oxyhemoglobin with mono-(3,5-dibromosalicyl)fumarate, produces a derivative specifically acylated at the two lysines β82, which can be purified with a 70% yield. The oxygen affinity of this derivative at 37°C at pH 7.4, 0.1 M Cl- is of 12 mm Hg, and is not affected by organic phosphate. In the presence of 5% CO2, the oxygen affinity decreases to 25 mm Hg. In all cases the cooperativity is lowered, with a value of n in the Hill plots near 2. Sedimentation velocity measurements indicate that, contrary to normal hemoglobin, this derivative fails to dissociate into dimers upon exposure to pH 5.5. The stability of the tetrameric structure is probably due to a modification of the β-β interface, resulting from electrostatic and hydrophobic interactions introduced in the β cleft by the fumaryl residues. These new interactions are probably the origin of a new reverse Bohr effect group at alkaline pH. Consistent with the stabilization of the tetrameric structure, the half-time of retention of this compound in the rat is increased 4-fold with respect to that of normal hemoglobin. These characteristics cast a favorable light on the usage of this compound as an oxygen carrier in transfusional and perfusional fluids.

Original languageEnglish (US)
Pages (from-to)6191-6195
Number of pages5
JournalJournal of Biological Chemistry
Volume264
Issue number11
StatePublished - 1989
Externally publishedYes

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Hemoglobins
Oxygen
Derivatives
Oxygen Compounds
Oxyhemoglobins
Organophosphates
Static Electricity
Hydrophobic and Hydrophilic Interactions
Sedimentation
Dimers
Velocity measurement
Lysine
Rats
Electrostatics
Stabilization
Light
Fluids
mono(3,5-dibromosalicyl)fumarate

ASJC Scopus subject areas

  • Biochemistry

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Pseudo cross-link of human hemoglobin with mono-(3,5-dibromosalicyl)fumarate. / Bucci, E.; Razynska, A.; Urbaitis, B.; Fronticelli, Clara.

In: Journal of Biological Chemistry, Vol. 264, No. 11, 1989, p. 6191-6195.

Research output: Contribution to journalArticle

Bucci, E. ; Razynska, A. ; Urbaitis, B. ; Fronticelli, Clara. / Pseudo cross-link of human hemoglobin with mono-(3,5-dibromosalicyl)fumarate. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 11. pp. 6191-6195.
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