Abstract
In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 61-68 |
| Number of pages | 8 |
| Journal | Journal of Biomedical Science |
| Volume | 4 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Mar 1997 |
Keywords
- CD34+ cells
- Hematopoiesis
- Microenvironment
- SCID mice
- Stromal cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)