Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice

S. Kaushal; V. F. La Russa; E. R. Hall; S. Gartner; J. H. Kim; L. P. Perera; Z. Yu; S. W. Kessler; J. D. Mosca

doi:10.1007/BF02255595

Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice

S. Kaushal, V. F. La Russa, E. R. Hall, S. Gartner, J. H. Kim, L. P. Perera, Z. Yu, S. W. Kessler, J. D. Mosca

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.

Original language	English (US)
Pages (from-to)	61-68
Number of pages	8
Journal	Journal of Biomedical Science
Volume	4
Issue number	2-3
DOIs	https://doi.org/10.1007/BF02255595
State	Published - Mar 1997

Keywords

CD34+ cells
Hematopoiesis
Microenvironment
SCID mice
Stromal cells

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/BF02255595

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title = "Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice",

abstract = "In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.",

keywords = "CD34+ cells, Hematopoiesis, Microenvironment, SCID mice, Stromal cells",

author = "S. Kaushal and {La Russa}, {V. F.} and Hall, {E. R.} and S. Gartner and Kim, {J. H.} and Perera, {L. P.} and Z. Yu and Kessler, {S. W.} and Mosca, {J. D.}",

year = "1997",

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doi = "10.1007/BF02255595",

language = "English (US)",

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AU - Kaushal, S.

AU - La Russa, V. F.

AU - Hall, E. R.

AU - Gartner, S.

AU - Kim, J. H.

AU - Perera, L. P.

AU - Yu, Z.

AU - Kessler, S. W.

AU - Mosca, J. D.

PY - 1997/3

Y1 - 1997/3

N2 - In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.

AB - In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.

KW - CD34+ cells

KW - Hematopoiesis

KW - Microenvironment

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KW - Stromal cells

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Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice

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Keywords

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