Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice

S. Kaushal, V. F. La Russa, E. R. Hall, S. Gartner, J. H. Kim, L. P. Perera, Z. Yu, S. W. Kessler, J. D. Mosca

Research output: Contribution to journalArticle

Abstract

In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalJournal of Biomedical Science
Volume4
Issue number2-3
DOIs
StatePublished - Mar 1997

Fingerprint

SCID Mice
Human Development
Bone
Animals
Cells
Bone Marrow
Gene therapy
Cell proliferation
Dosimetry
Animal Models
Interleukin-6
Cell Line
Irradiation
Cytokines
Plasmas
Stromal Cells
Growth
Intraperitoneal Injections
Intravenous Injections
Bone Marrow Cells

Keywords

  • CD34+ cells
  • Hematopoiesis
  • Microenvironment
  • SCID mice
  • Stromal cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kaushal, S., La Russa, V. F., Hall, E. R., Gartner, S., Kim, J. H., Perera, L. P., ... Mosca, J. D. (1997). Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice. Journal of Biomedical Science, 4(2-3), 61-68. https://doi.org/10.1007/BF02255595

Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice. / Kaushal, S.; La Russa, V. F.; Hall, E. R.; Gartner, S.; Kim, J. H.; Perera, L. P.; Yu, Z.; Kessler, S. W.; Mosca, J. D.

In: Journal of Biomedical Science, Vol. 4, No. 2-3, 03.1997, p. 61-68.

Research output: Contribution to journalArticle

Kaushal, S, La Russa, VF, Hall, ER, Gartner, S, Kim, JH, Perera, LP, Yu, Z, Kessler, SW & Mosca, JD 1997, 'Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice', Journal of Biomedical Science, vol. 4, no. 2-3, pp. 61-68. https://doi.org/10.1007/BF02255595
Kaushal, S. ; La Russa, V. F. ; Hall, E. R. ; Gartner, S. ; Kim, J. H. ; Perera, L. P. ; Yu, Z. ; Kessler, S. W. ; Mosca, J. D. / Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice. In: Journal of Biomedical Science. 1997 ; Vol. 4, No. 2-3. pp. 61-68.
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