Proton NMR spectroscopy of solvent-saturable resonances: A new approach to study pH effects in situ

Susumu Mori, Scott M. Eleff, Ulrich Pilatus, Noriko Mori, Peter C.M. Van Zijl

Research output: Contribution to journalArticle

Abstract

It is shown that the effect of pH changes can be measured in proton NMR spectra through the pH sensitivity of the signal intensities of metabolite protons exchanging with water. To observe this phenomenon, pulse sequences must be used that can sensitively observe these exchangeable protons under physiological conditions, which is achieved by avoiding magnetization transfer signal losses due to water saturation for solvent suppression purposes, These methods provide an order-of-magnitude enhancement of many signals between 5 and 10 ppm, containing both N-bound protons as well as aromatic C-H protons coupled to them, the intensity of which is influenced by exchange-relayed saturation. As a first application, the effects of pH change on these resonances are studied ex vivo (perfused cells) and in vivo (cat brain).

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalMagnetic resonance in medicine
Volume40
Issue number1
DOIs
StatePublished - Jul 1 1998

Keywords

  • Cat brain
  • Exchangeable protons
  • Perfused cells
  • Saturation transfer

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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