Protocol for mosquito rearing (A. gambiae)

Suchismita Das, Lindsey Garver, George Dimopoulos

Research output: Contribution to journalArticle

Abstract

This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28°C and ∼80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosquito food (described below), mosquito net to cover the trays, vacuum, and a collection chamber to collect adults.

Original languageEnglish (US)
Article numbere221
JournalJournal of Visualized Experiments
Issue number5
DOIs
StatePublished - Jun 2007

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Culicidae
Water
Sugar (sucrose)
Mosquito Nets
Paraffin
Sucrose
Atmospheric humidity
Blood
Vacuum
Plastics
Humidity
Baths
Glass
Food
Serum

Keywords

  • Cellular biology
  • Infectious disease
  • Issue 5
  • Malaria
  • Mosquito

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Protocol for mosquito rearing (A. gambiae). / Das, Suchismita; Garver, Lindsey; Dimopoulos, George.

In: Journal of Visualized Experiments, No. 5, e221, 06.2007.

Research output: Contribution to journalArticle

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