Protocol for mosquito rearing (A. gambiae)

Suchismita Das; Lindsey Garver; George Dimopoulos

doi:10.3791/221

Protocol for mosquito rearing (A. gambiae)

Suchismita Das, Lindsey Garver, George Dimopoulos

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28°C and ∼80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosquito food (described below), mosquito net to cover the trays, vacuum, and a collection chamber to collect adults.

Original language	English (US)
Article number	e221
Journal	Journal of Visualized Experiments
Issue number	5
DOIs	https://doi.org/10.3791/221
State	Published - Jun 2007

Keywords

Cellular biology
Infectious disease
Issue 5
Malaria
Mosquito

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.3791/221

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Protocol for mosquito rearing (A. gambiae)

Abstract

Keywords

ASJC Scopus subject areas

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