Abstract
This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28°C and ∼80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosquito food (described below), mosquito net to cover the trays, vacuum, and a collection chamber to collect adults.
| Original language | English (US) |
|---|---|
| Article number | e221 |
| Journal | Journal of Visualized Experiments |
| Issue number | 5 |
| DOIs | |
| State | Published - Jun 1 2007 |
Keywords
- Cellular biology
- Infectious disease
- Issue 5
- Malaria
- Mosquito
ASJC Scopus subject areas
- Neuroscience(all)
- Chemical Engineering(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
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Cite this
Das, S., Garver, L., & Dimopoulos, G. (2007). Protocol for mosquito rearing (A. gambiae). Journal of Visualized Experiments, (5), [e221]. https://doi.org/10.3791/221