Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons

Xiling Yin, Jungwoo Wren Kim, Shiyu Liu, Ted M. Dawson, Valina L. Dawson

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium regulation is a critical process in neurons, and Ca2+ signaling is a major contributor to neurological disorders including Parkinson's disease (PD). Here, combining calcium imaging with whole-cell Ca2+ current recording, we provide a detailed protocol for measuring Ca2+ homeostasis in dopaminergic (DA) neurons derived from human induced pluripotent stem cells (hiPSCs). This approach can be applied to investigate the role of Ca2+ homeostasis in neuronal functionality as well as in disease processes. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

Original languageEnglish (US)
Article number100405
JournalSTAR Protocols
Volume2
Issue number2
DOIs
StatePublished - Jun 18 2021

Keywords

  • Cell Differentiation
  • Microscopy
  • Neuroscience
  • Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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