Protocol for detection of hiv-tat protein in cerebrospinal fluid by a sandwich enzyme-linked immunosorbent assay

Tory P. Johnson, Avindra Nath

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The human immunodefi ciency virus (HIV) transactivator of transcription (Tat) is a virally produced protein that is required for effi cient viral replication. Once formed inside an infected cell, Tat is secreted into the extracellular space where it has pathophysiological consequences on cells it interacts with. Tat has been demonstrated to be neurotoxic and is produced even under the pressures of anti-retroviral therapy;therefore Tat is suspected to contribute to the development of HIV-associated neurocognitive disorders. In this chapter, we describe a sandwich enzyme-linked immunosorbent assay protocol for the detection of Tat from cerebrospinal fl uid samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages343-352
Number of pages10
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)1064-3745

Keywords

  • CSF
  • Cell lysates
  • ELISA
  • HIV
  • Protein detection
  • Tat
  • Transactivator of transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Johnson, T. P., & Nath, A. (2016). Protocol for detection of hiv-tat protein in cerebrospinal fluid by a sandwich enzyme-linked immunosorbent assay. In Methods in Molecular Biology (pp. 343-352). (Methods in Molecular Biology; Vol. 1354). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3046-3_23