Protocol for dengue infections in mosquitoes (A. aegypti) and infection phenotype determination

Suchismita Das, Lindsey Garver, Jose Ruiz Ramirez, Zhiyong Xi, George Dimopoulos

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of the virus in the mosquito tissues. This protocol is routinely used for studying mosquito-virus interactions, especially for identification of novel host factors that are able to determine vector competence. The entire experiment must be conducted in a BSL2 laboratory. Similar to Plasmodium falciparum infections, proper attire including gloves and lab coat must be worn at all times. After the experiment, all the materials that came in contact with the virus need to be treated with 75% ethanol and bleached before proceeding with normal washing. All other materials need to be autoclaved before discarding them.

Original languageEnglish (US)
Article numbere220
JournalJournal of Visualized Experiments
Issue number5
DOIs
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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