Heng Zhu, Metin Bilgin, Michael Snyder

Research output: Contribution to journalReview articlepeer-review


Fueled by ever-growing DNA sequence information, proteomics - the large scale analysis of proteins - has become one of the most important disciplines for characterizing gene function, for building functional linkages between protein molecules, and for providing insight into the mechanisms of biological processes in a high-throughput mode. It is now possible to examine the expression of more than 1000 proteins using mass spectrometry technology coupled with various separation methods. High-throughput yeast two-hybrid approaches and analysis of protein complexes using affinity tag purification have yielded valuable protein-protein interaction maps. Large-scale protein tagging and subcellular localization projects have provided considerable information about protein function. Finally, recent developments in protein microarray technology provide a versatile tool to study protein-protein, protein-nucleic acid, protein-lipid, enzyme-substrate, and protein-drug interactions. Other types of microarrays, though not fully developed, also show great potential in diagnostics, protein profiling, and drug identification and validation. This review discusses high-throughput technologies for proteome analysis and their applications. Also discussed are the approaches used for the integrated analysis of the voluminous sets of data generated by proteome analysis conducted on a global scale.

Original languageEnglish (US)
Pages (from-to)783-812
Number of pages30
JournalAnnual review of biochemistry
StatePublished - 2003
Externally publishedYes


  • 2-D/MS
  • Data integration
  • Protein localization
  • Proteome microarray
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biochemistry


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