Proteomic profiling of acute cardiac allograft rejection

Katrin Kienzl, Bettina Sarg, Georg Golderer, Peter Obrist, Ernst R. Werner, Gabriele Werner-Felmayer, Herbert Lindner, Manuel Maglione, Stefan Schneeberger, Raimund Margreiter, Gerald Brandacher

Research output: Contribution to journalArticle

Abstract

Background.: Proteome analysis has emerged as a valuable tool for the study of large-scale protein expression profiles. Here, we applied this novel technology to identify specific biomarkers for acute cardiac allograft rejection. Methods.: Hearts of C57BL/10 mice were placed in fully major histocompatibility complex-mismatched C3H/He recipients. Syngeneic transplants served as controls. Intragraft protein expression analysis was performed using fluorescence two-dimensional difference gel electrophoresis on day 6 posttransplant. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. In addition, expression of selected proteins was confirmed by Western blot analysis and by immunohistochemistry. Results.: Two-dimensional difference gel electrophoresis enabled detection of 1541 protein spots. For 95 protein spots, the expression level during acute rejection differed by more than 1.5-fold from that observed in syngeneic grafts. Spots with significant differential regulation identified by tandem mass spectrometry were derived from peroxiredoxin 6, pyruvate kinase isozyme M2, coronin 1A, protein disulfide isomerase A3 precursor, and aconitate hydratase. Conclusion.: These identified proteins may constitute novel biomarkers of acute cardiac allograft rejection and might hold great potential as surrogate markers for monitoring in vivo alloimmune response.

Original languageEnglish (US)
Pages (from-to)553-560
Number of pages8
JournalTransplantation
Volume88
Issue number4
DOIs
StatePublished - Aug 2009
Externally publishedYes

Fingerprint

Proteomics
Allografts
Two-Dimensional Difference Gel Electrophoresis
Proteins
Biomarkers
Tandem Mass Spectrometry
Peroxiredoxin VI
Aconitate Hydratase
Protein Disulfide-Isomerases
Transplants
Pyruvate Kinase
Proteome
Major Histocompatibility Complex
Inbred C57BL Mouse
Isoenzymes
Fluorescence
Western Blotting
Immunohistochemistry
Technology

Keywords

  • Biomarker.
  • Heart transplantation
  • Proteomics

ASJC Scopus subject areas

  • Transplantation

Cite this

Kienzl, K., Sarg, B., Golderer, G., Obrist, P., Werner, E. R., Werner-Felmayer, G., ... Brandacher, G. (2009). Proteomic profiling of acute cardiac allograft rejection. Transplantation, 88(4), 553-560. https://doi.org/10.1097/TP.0b013e3181b119b1

Proteomic profiling of acute cardiac allograft rejection. / Kienzl, Katrin; Sarg, Bettina; Golderer, Georg; Obrist, Peter; Werner, Ernst R.; Werner-Felmayer, Gabriele; Lindner, Herbert; Maglione, Manuel; Schneeberger, Stefan; Margreiter, Raimund; Brandacher, Gerald.

In: Transplantation, Vol. 88, No. 4, 08.2009, p. 553-560.

Research output: Contribution to journalArticle

Kienzl, K, Sarg, B, Golderer, G, Obrist, P, Werner, ER, Werner-Felmayer, G, Lindner, H, Maglione, M, Schneeberger, S, Margreiter, R & Brandacher, G 2009, 'Proteomic profiling of acute cardiac allograft rejection', Transplantation, vol. 88, no. 4, pp. 553-560. https://doi.org/10.1097/TP.0b013e3181b119b1
Kienzl K, Sarg B, Golderer G, Obrist P, Werner ER, Werner-Felmayer G et al. Proteomic profiling of acute cardiac allograft rejection. Transplantation. 2009 Aug;88(4):553-560. https://doi.org/10.1097/TP.0b013e3181b119b1
Kienzl, Katrin ; Sarg, Bettina ; Golderer, Georg ; Obrist, Peter ; Werner, Ernst R. ; Werner-Felmayer, Gabriele ; Lindner, Herbert ; Maglione, Manuel ; Schneeberger, Stefan ; Margreiter, Raimund ; Brandacher, Gerald. / Proteomic profiling of acute cardiac allograft rejection. In: Transplantation. 2009 ; Vol. 88, No. 4. pp. 553-560.
@article{6bd2109c31824ac6887909ceb839f7ae,
title = "Proteomic profiling of acute cardiac allograft rejection",
abstract = "Background.: Proteome analysis has emerged as a valuable tool for the study of large-scale protein expression profiles. Here, we applied this novel technology to identify specific biomarkers for acute cardiac allograft rejection. Methods.: Hearts of C57BL/10 mice were placed in fully major histocompatibility complex-mismatched C3H/He recipients. Syngeneic transplants served as controls. Intragraft protein expression analysis was performed using fluorescence two-dimensional difference gel electrophoresis on day 6 posttransplant. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. In addition, expression of selected proteins was confirmed by Western blot analysis and by immunohistochemistry. Results.: Two-dimensional difference gel electrophoresis enabled detection of 1541 protein spots. For 95 protein spots, the expression level during acute rejection differed by more than 1.5-fold from that observed in syngeneic grafts. Spots with significant differential regulation identified by tandem mass spectrometry were derived from peroxiredoxin 6, pyruvate kinase isozyme M2, coronin 1A, protein disulfide isomerase A3 precursor, and aconitate hydratase. Conclusion.: These identified proteins may constitute novel biomarkers of acute cardiac allograft rejection and might hold great potential as surrogate markers for monitoring in vivo alloimmune response.",
keywords = "Biomarker., Heart transplantation, Proteomics",
author = "Katrin Kienzl and Bettina Sarg and Georg Golderer and Peter Obrist and Werner, {Ernst R.} and Gabriele Werner-Felmayer and Herbert Lindner and Manuel Maglione and Stefan Schneeberger and Raimund Margreiter and Gerald Brandacher",
year = "2009",
month = "8",
doi = "10.1097/TP.0b013e3181b119b1",
language = "English (US)",
volume = "88",
pages = "553--560",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Proteomic profiling of acute cardiac allograft rejection

AU - Kienzl, Katrin

AU - Sarg, Bettina

AU - Golderer, Georg

AU - Obrist, Peter

AU - Werner, Ernst R.

AU - Werner-Felmayer, Gabriele

AU - Lindner, Herbert

AU - Maglione, Manuel

AU - Schneeberger, Stefan

AU - Margreiter, Raimund

AU - Brandacher, Gerald

PY - 2009/8

Y1 - 2009/8

N2 - Background.: Proteome analysis has emerged as a valuable tool for the study of large-scale protein expression profiles. Here, we applied this novel technology to identify specific biomarkers for acute cardiac allograft rejection. Methods.: Hearts of C57BL/10 mice were placed in fully major histocompatibility complex-mismatched C3H/He recipients. Syngeneic transplants served as controls. Intragraft protein expression analysis was performed using fluorescence two-dimensional difference gel electrophoresis on day 6 posttransplant. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. In addition, expression of selected proteins was confirmed by Western blot analysis and by immunohistochemistry. Results.: Two-dimensional difference gel electrophoresis enabled detection of 1541 protein spots. For 95 protein spots, the expression level during acute rejection differed by more than 1.5-fold from that observed in syngeneic grafts. Spots with significant differential regulation identified by tandem mass spectrometry were derived from peroxiredoxin 6, pyruvate kinase isozyme M2, coronin 1A, protein disulfide isomerase A3 precursor, and aconitate hydratase. Conclusion.: These identified proteins may constitute novel biomarkers of acute cardiac allograft rejection and might hold great potential as surrogate markers for monitoring in vivo alloimmune response.

AB - Background.: Proteome analysis has emerged as a valuable tool for the study of large-scale protein expression profiles. Here, we applied this novel technology to identify specific biomarkers for acute cardiac allograft rejection. Methods.: Hearts of C57BL/10 mice were placed in fully major histocompatibility complex-mismatched C3H/He recipients. Syngeneic transplants served as controls. Intragraft protein expression analysis was performed using fluorescence two-dimensional difference gel electrophoresis on day 6 posttransplant. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. In addition, expression of selected proteins was confirmed by Western blot analysis and by immunohistochemistry. Results.: Two-dimensional difference gel electrophoresis enabled detection of 1541 protein spots. For 95 protein spots, the expression level during acute rejection differed by more than 1.5-fold from that observed in syngeneic grafts. Spots with significant differential regulation identified by tandem mass spectrometry were derived from peroxiredoxin 6, pyruvate kinase isozyme M2, coronin 1A, protein disulfide isomerase A3 precursor, and aconitate hydratase. Conclusion.: These identified proteins may constitute novel biomarkers of acute cardiac allograft rejection and might hold great potential as surrogate markers for monitoring in vivo alloimmune response.

KW - Biomarker.

KW - Heart transplantation

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=70349123528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349123528&partnerID=8YFLogxK

U2 - 10.1097/TP.0b013e3181b119b1

DO - 10.1097/TP.0b013e3181b119b1

M3 - Article

C2 - 19696639

AN - SCOPUS:70349123528

VL - 88

SP - 553

EP - 560

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 4

ER -