Proteomic composition and immunomodulatory properties of urinary bladder matrix scaffolds in homeostasis and injury

Kaitlyn Sadtler, Sven D. Sommerfeld, Matthew T. Wolf, Xiaokun Wang, Shoumyo Majumdar, Liam Chung, Dhanashree S. Kelkar, Akhilesh Pandey, Jennifer H. Elisseeff

Research output: Contribution to journalArticle

Abstract

Urinary bladder matrix (UBM) is used clinically for management of wounds and reinforcement of surgical soft tissue repair, among other applications. UBM consists of the lamina propria and basal lamina of the porcine urinary bladder, and is decellularized as part of the process to manufacture the medical device. UBM is composed mainly of Collagen I, but also contains a wide variety of fibrillar and basement membrane collagens, glycoproteins, proteoglycans and ECM-associated factors. Upon application of the biomaterial in a traumatic or non-traumatic setting in a mouse model, there is a cascade of immune cells that respond to the damaged tissue and biomaterial. Here, through the use of multicolor flow cytometry, we describe the various cells that infiltrate the UBM scaffold in a subcutaneous and volumetric muscle injury model. A wide variety of immune cells are found in the UBM scaffold immune microenvironment (SIM) including F4/80+ macrophages, CD11c+ dendritic cells, CD3+ T cells and CD19+ B cells. A systemic IL-4 upregulation and a local M2-macrophage response were observed in the proximity of the implanted UBM. The recruitment and activation of these cells is dependent upon signals from the scaffold and communication between the different cell types present.

Original languageEnglish (US)
JournalSeminars in Immunology
DOIs
StateAccepted/In press - Feb 28 2017

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Proteomics
Urinary Bladder
Homeostasis
Wounds and Injuries
Biocompatible Materials
Basement Membrane
Collagen
Macrophages
Membrane Glycoproteins
Proteoglycans
Interleukin-4
Dendritic Cells
Flow Cytometry
Mucous Membrane
B-Lymphocytes
Up-Regulation
Swine
Communication
T-Lymphocytes
Equipment and Supplies

Keywords

  • Biomaterials
  • Immunology
  • Macrophages
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Proteomic composition and immunomodulatory properties of urinary bladder matrix scaffolds in homeostasis and injury. / Sadtler, Kaitlyn; Sommerfeld, Sven D.; Wolf, Matthew T.; Wang, Xiaokun; Majumdar, Shoumyo; Chung, Liam; Kelkar, Dhanashree S.; Pandey, Akhilesh; Elisseeff, Jennifer H.

In: Seminars in Immunology, 28.02.2017.

Research output: Contribution to journalArticle

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