Proteomic approach for identification of serine/threonine phosphorylated proteins

Mads Grønborg, Troels Z. Kristiansen, Allan Stensballe, Jens S. Andersen, Matthias Mann, Akhilesh Pandey, Ole N. Jensen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages309-310
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Threonine
Serine
Phosphothreonine
Phosphoserine
Proteins
Antibodies
Phosphoproteins
Display devices
Proteomics

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Grønborg, M., Kristiansen, T. Z., Stensballe, A., Andersen, J. S., Mann, M., Pandey, A., & Jensen, O. N. (2002). Proteomic approach for identification of serine/threonine phosphorylated proteins. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 309-310)

Proteomic approach for identification of serine/threonine phosphorylated proteins. / Grønborg, Mads; Kristiansen, Troels Z.; Stensballe, Allan; Andersen, Jens S.; Mann, Matthias; Pandey, Akhilesh; Jensen, Ole N.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 309-310.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Grønborg, M, Kristiansen, TZ, Stensballe, A, Andersen, JS, Mann, M, Pandey, A & Jensen, ON 2002, Proteomic approach for identification of serine/threonine phosphorylated proteins. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 309-310, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Grønborg M, Kristiansen TZ, Stensballe A, Andersen JS, Mann M, Pandey A et al. Proteomic approach for identification of serine/threonine phosphorylated proteins. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 309-310
Grønborg, Mads ; Kristiansen, Troels Z. ; Stensballe, Allan ; Andersen, Jens S. ; Mann, Matthias ; Pandey, Akhilesh ; Jensen, Ole N. / Proteomic approach for identification of serine/threonine phosphorylated proteins. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 309-310
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