Proteomic approach for identification of serine/threonine phosphorylated proteins

Mads Grønborg; Troels Z. Kristiansen; Allan Stensballe; Jens S. Andersen; Matthias Mann; Akhilesh Pandey; Ole N. Jensen

Proteomic approach for identification of serine/threonine phosphorylated proteins

Mads Grønborg, Troels Z. Kristiansen, Allan Stensballe, Jens S. Andersen, Matthias Mann, Akhilesh Pandey, Ole N. Jensen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	309-310
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Grønborg, M, Kristiansen, TZ, Stensballe, A, Andersen, JS, Mann, M, Pandey, A & Jensen, ON 2002, Proteomic approach for identification of serine/threonine phosphorylated proteins. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 309-310, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

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abstract = "Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.",

author = "Mads Gr{\o}nborg and Kristiansen, {Troels Z.} and Allan Stensballe and Andersen, {Jens S.} and Matthias Mann and Akhilesh Pandey and Jensen, {Ole N.}",

year = "2002",

language = "English (US)",

pages = "309--310",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - GEN

T1 - Proteomic approach for identification of serine/threonine phosphorylated proteins

AU - Grønborg, Mads

AU - Kristiansen, Troels Z.

AU - Stensballe, Allan

AU - Andersen, Jens S.

AU - Mann, Matthias

AU - Pandey, Akhilesh

AU - Jensen, Ole N.

PY - 2002

Y1 - 2002

N2 - Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.

AB - Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.

UR - http://www.scopus.com/inward/record.url?scp=2442665658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442665658&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442665658

SP - 309

EP - 310

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Proteomic approach for identification of serine/threonine phosphorylated proteins

Abstract

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ASJC Scopus subject areas

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