Proteomic and histochemical analysis of proteins involved in the dying-back-type of axonal degeneration in the gracile axonal dystrophy (gad) mouse

Akiko Goto, Yu Lai Wang, Tomohiro Kabuta, Rieko Setsuie, Hitoshi Osaka, Akira Sawa, Shoichi Ishiura, Keiji Wada

Research output: Contribution to journalArticle

Abstract

Local axonal degeneration is a common pathological feature of peripheral neuropathies and neurodegenerative disorders of the central nervous system, including Alzheimer's disease, Parkinson's disease, and stroke; however, the underlying molecular mechanism is not known. Here, we analyzed the gracile axonal dystrophy (gad) mouse, which displays the dying-back-type of axonal degeneration in sensory neurons, to find the molecules involved in the mechanism of axonal degeneration. The gad mouse is analogous to a null mutant of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1). UCH-L1 is a deubiquitinating enzyme expressed at high levels in neurons, as well as testis and ovary. In addition, we recently discovered a new function of UCH-L1-namely to bind to and stabilize mono-ubiquitin in neurons, and found that the level of mono-ubiquitin was decreased in neurons, especially in axons of the sciatic nerve, in gad mice. The low level of ubiquitin suggests that the target proteins of the ubiquitin proteasome system are not sufficiently ubiquitinated and thus degraded in the gad mouse; therefore, these proteins may be the key molecules involved in axonal degeneration. To identify molecules involved in axonal degeneration in gad mice, we compared protein expression in sciatic nerves between gad and wild-type mice at 2 and 12 weeks old, using two-dimensional difference gel electrophoresis. As a result, we found age-dependent accumulation of several proteins, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 14-3-3, in gad mice compared with wild-type mice. Histochemical analyses demonstrated that GAPDH and 14-3-3 were localized throughout axons in both gad and wild-type mice, but GAPDH accumulated in the axons of gad mice. Recently, it has been suggested that a wide range of neurodegenerative diseases are characterized by the accumulation of intracellular and extracellular protein aggregates, and it has been reported that oxidative stress causes the aggregation of GAPDH. Furthermore, histochemical analysis demonstrated that sulfonated GAPDH, a sensor of oxidative stress that elicits cellular dysfunction, was expressed in the axons of gad mice, and 4-hydroxy-2-nonenal, a major marker of oxidative stress, was also only detected in gad mice. Our findings suggest that GAPDH may participate in a process of the dying-back-type of axonal degeneration in gad mice and may provide valuable insight into the mechanisms of axonal degeneration.

Original languageEnglish (US)
Pages (from-to)330-338
Number of pages9
JournalNeurochemistry International
Volume54
Issue number5-6
DOIs
StatePublished - May 2009

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Proteomics
Glyceraldehyde-3-Phosphate Dehydrogenases
Ubiquitin
Proteins
Axons
Hydrolases
Oxidative Stress
Sciatic Nerve
Neurons
Neurodegenerative Diseases
Two-Dimensional Difference Gel Electrophoresis
Peripheral Nervous System Diseases
Sensory Receptor Cells
Proteasome Endopeptidase Complex
Parkinson Disease
Testis
Ovary
Alzheimer Disease
Central Nervous System
Stroke

Keywords

  • 2D-DIGE
  • Axonal degeneration
  • Dying-back
  • gad mouse
  • GAPDH
  • Oxidative stress
  • Ubiquitin
  • UCH-L1

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Proteomic and histochemical analysis of proteins involved in the dying-back-type of axonal degeneration in the gracile axonal dystrophy (gad) mouse. / Goto, Akiko; Wang, Yu Lai; Kabuta, Tomohiro; Setsuie, Rieko; Osaka, Hitoshi; Sawa, Akira; Ishiura, Shoichi; Wada, Keiji.

In: Neurochemistry International, Vol. 54, No. 5-6, 05.2009, p. 330-338.

Research output: Contribution to journalArticle

Goto, Akiko ; Wang, Yu Lai ; Kabuta, Tomohiro ; Setsuie, Rieko ; Osaka, Hitoshi ; Sawa, Akira ; Ishiura, Shoichi ; Wada, Keiji. / Proteomic and histochemical analysis of proteins involved in the dying-back-type of axonal degeneration in the gracile axonal dystrophy (gad) mouse. In: Neurochemistry International. 2009 ; Vol. 54, No. 5-6. pp. 330-338.
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