Proteomic analysis of the rice seed for quality improvement

Y. J. Kim, S. H. Choi, Bongsoo Park, J. T. Song, M. C. Kim, H. J. Koh, H. S. Seo

Research output: Contribution to journalArticle

Abstract

The amount, composition and distribution of seed storage proteins in rice are important factors for flavour and quality. We have analysed the seed proteomes of a relatively high-quality cultivar, 'Gopum' and a relatively low-quality cultivar, 'Dobong. By two-dimensional gel electrophoresis and subsequent MALDI-TOF/MS, we identified 'Gopum'- and 'Dobong'-specific seed proteins at 15 or 50 days after flowering. Of 53 identified proteins, we chose 15 that may have important roles in quality determination or maintenance through regulation of protein stability or disease resistance during grain filling and storage. By real-time (RT)-PCR, the expression of a few proteins tightly correlated with transcript levels at specific stages, while that of others did not. Our results suggest that levels of the identified proteins are determined post-transcriptionally or post-translationally, and that proteins which were expressed in the seeds of either 'Gopum' or 'Dobong' may affect rice flavour and quality.

Original languageEnglish (US)
Pages (from-to)541-550
Number of pages10
JournalPlant Breeding
Volume128
Issue number6
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

seed quality
Quality Improvement
Proteomics
proteomics
Seeds
rice
Proteins
proteins
flavor
seeds
Seed Storage Proteins
Disease Resistance
seed storage proteins
Protein Stability
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
cultivars
Proteome
proteome

Keywords

  • Dobong
  • Endosperm
  • Flavour
  • Gopum
  • Protein
  • Quality

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Kim, Y. J., Choi, S. H., Park, B., Song, J. T., Kim, M. C., Koh, H. J., & Seo, H. S. (2009). Proteomic analysis of the rice seed for quality improvement. Plant Breeding, 128(6), 541-550. https://doi.org/10.1111/j.1439-0523.2009.01693.x

Proteomic analysis of the rice seed for quality improvement. / Kim, Y. J.; Choi, S. H.; Park, Bongsoo; Song, J. T.; Kim, M. C.; Koh, H. J.; Seo, H. S.

In: Plant Breeding, Vol. 128, No. 6, 12.2009, p. 541-550.

Research output: Contribution to journalArticle

Kim, YJ, Choi, SH, Park, B, Song, JT, Kim, MC, Koh, HJ & Seo, HS 2009, 'Proteomic analysis of the rice seed for quality improvement', Plant Breeding, vol. 128, no. 6, pp. 541-550. https://doi.org/10.1111/j.1439-0523.2009.01693.x
Kim, Y. J. ; Choi, S. H. ; Park, Bongsoo ; Song, J. T. ; Kim, M. C. ; Koh, H. J. ; Seo, H. S. / Proteomic analysis of the rice seed for quality improvement. In: Plant Breeding. 2009 ; Vol. 128, No. 6. pp. 541-550.
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