Proteomic analysis of the mammalian nuclear pore complex

Janet M. Cronshaw, Andrew N. Krutchinsky, Wenzhu Zhang, Brian T. Chait, Michael J Matunis

Research output: Contribution to journalArticle

Abstract

As the sole site of nucleocytoplasmic transport, the nuclear pore complex (NPC) has a vital cellular role. Nonetheless, much remains to be learned about many fundamental aspects of NPC function. To further understand the structure and function of the mammalian NPC, we have completed a proteomic analysis to identify and classify all of its protein components. We used mass spectrometry to identify all proteins present in a biochemically purified NPC fraction. Based on previous characterization, sequence homology, and subcellular localization, 29 of these proteins were classified as nucleoporins, and a further 18 were classified as NPC-associated proteins. Among the 29 nucleoporins were six previously undiscovered nucleoporins and a novel family of WD repeat nucleoporins. One of these WD repeat nucleoporins is ALADIN, the gene mutated in triple-A (or Allgrove) syndrome. Our analysis defines the proteome of the mammalian NPC for the first time and paves the way for a more detailed characterization of NPC structure and function.

Original languageEnglish (US)
Pages (from-to)915-927
Number of pages13
JournalJournal of Cell Biology
Volume158
Issue number5
DOIs
StatePublished - Sep 2 2002

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Nuclear Pore Complex Proteins
Nuclear Pore
Proteomics
Proteins
Cell Nucleus Active Transport
Proteome
Sequence Homology
Mass Spectrometry
Genes

Keywords

  • Nuclear pore complex
  • Nucleocytoplasmic transport
  • Nucleoporins
  • Proteomics
  • WD repeats

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cronshaw, J. M., Krutchinsky, A. N., Zhang, W., Chait, B. T., & Matunis, M. J. (2002). Proteomic analysis of the mammalian nuclear pore complex. Journal of Cell Biology, 158(5), 915-927. https://doi.org/10.1083/jcb.200206106

Proteomic analysis of the mammalian nuclear pore complex. / Cronshaw, Janet M.; Krutchinsky, Andrew N.; Zhang, Wenzhu; Chait, Brian T.; Matunis, Michael J.

In: Journal of Cell Biology, Vol. 158, No. 5, 02.09.2002, p. 915-927.

Research output: Contribution to journalArticle

Cronshaw, JM, Krutchinsky, AN, Zhang, W, Chait, BT & Matunis, MJ 2002, 'Proteomic analysis of the mammalian nuclear pore complex', Journal of Cell Biology, vol. 158, no. 5, pp. 915-927. https://doi.org/10.1083/jcb.200206106
Cronshaw, Janet M. ; Krutchinsky, Andrew N. ; Zhang, Wenzhu ; Chait, Brian T. ; Matunis, Michael J. / Proteomic analysis of the mammalian nuclear pore complex. In: Journal of Cell Biology. 2002 ; Vol. 158, No. 5. pp. 915-927.
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