TY - JOUR
T1 - Proteomic analysis of the mammalian nuclear pore complex
AU - Cronshaw, Janet M.
AU - Krutchinsky, Andrew N.
AU - Zhang, Wenzhu
AU - Chait, Brian T.
AU - Matunis, Michae L.J.
PY - 2002/9/2
Y1 - 2002/9/2
N2 - As the sole site of nucleocytoplasmic transport, the nuclear pore complex (NPC) has a vital cellular role. Nonetheless, much remains to be learned about many fundamental aspects of NPC function. To further understand the structure and function of the mammalian NPC, we have completed a proteomic analysis to identify and classify all of its protein components. We used mass spectrometry to identify all proteins present in a biochemically purified NPC fraction. Based on previous characterization, sequence homology, and subcellular localization, 29 of these proteins were classified as nucleoporins, and a further 18 were classified as NPC-associated proteins. Among the 29 nucleoporins were six previously undiscovered nucleoporins and a novel family of WD repeat nucleoporins. One of these WD repeat nucleoporins is ALADIN, the gene mutated in triple-A (or Allgrove) syndrome. Our analysis defines the proteome of the mammalian NPC for the first time and paves the way for a more detailed characterization of NPC structure and function.
AB - As the sole site of nucleocytoplasmic transport, the nuclear pore complex (NPC) has a vital cellular role. Nonetheless, much remains to be learned about many fundamental aspects of NPC function. To further understand the structure and function of the mammalian NPC, we have completed a proteomic analysis to identify and classify all of its protein components. We used mass spectrometry to identify all proteins present in a biochemically purified NPC fraction. Based on previous characterization, sequence homology, and subcellular localization, 29 of these proteins were classified as nucleoporins, and a further 18 were classified as NPC-associated proteins. Among the 29 nucleoporins were six previously undiscovered nucleoporins and a novel family of WD repeat nucleoporins. One of these WD repeat nucleoporins is ALADIN, the gene mutated in triple-A (or Allgrove) syndrome. Our analysis defines the proteome of the mammalian NPC for the first time and paves the way for a more detailed characterization of NPC structure and function.
KW - Nuclear pore complex
KW - Nucleocytoplasmic transport
KW - Nucleoporins
KW - Proteomics
KW - WD repeats
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U2 - 10.1083/jcb.200206106
DO - 10.1083/jcb.200206106
M3 - Article
C2 - 12196509
AN - SCOPUS:0037008997
SN - 0021-9525
VL - 158
SP - 915
EP - 927
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -