Proteomic analysis of the dorsal spinal cord in the mouse model of spared nerve injury-induced neuropathic pain

Eun Sung Park, Jung Mo Ahn, Sang Min Jeon, Hee Jung Cho, Ki Myung Chung, Je Yoel Cho, Dong Ho Youn

Research output: Contribution to journalArticle

Abstract

Peripheral nerve injury often causes neuropathic pain and is associated with changes in the expression of numerous proteins in the dorsal horn of the spinal cord. To date, proteomic analysis method has been used to simultaneously analyze hundreds or thousands of proteins differentially expressed in the dorsal horn of the spinal cord in rats or dorsal root ganglion of rats with certain type of peripheral nerve injury. However, a proteomic study using a mouse model of neuropathic pain could be attempted because of abundant protein database and the availability of transgenic mice. In this study, whole proteins were extracted from the ipsilateral dorsal half of the 4th-6th lumbar spinal cord in a mouse model of spared nerve injury (SNI)-induced neuropathic pain. In-gel digests of the proteins size-separated on a polyacrylamide gel were subjected to reverse-phase liquid-chromatography coupled with electrospray ionization ion trap tandem mass spectrometry (MS/MS). After identifying proteins, the data were analyzed with subtractive proteomics using ProtAn, an in-house analytic program. Consequently, 15 downregulated and 35 upregulated proteins were identified in SNI mice. The identified proteins may contribute to the maintenance of neuropathic pain, and may provide new or valuable information in the discovery of new therapeutic targets for neuropathic pain.

Original languageEnglish (US)
Pages (from-to)494-502
Number of pages9
JournalJournal of Biomedical Research
Volume31
Issue number6
DOIs
StatePublished - 2017

Keywords

  • Mouse
  • Neuropathic pain
  • Proteomics
  • Spared nerve injury
  • Spinal dorsal horn

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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