TY - JOUR
T1 - Proteomic analysis of ribosomes
T2 - Translational control of mRNA populations by glycogen synthase GYS1
AU - Fuchs, Gabriele
AU - Diges, Camille
AU - Kohlstaedt, Lori A.
AU - Wehner, Karen A.
AU - Sarnow, Peter
N1 - Funding Information:
We thank Karla Kirkegaard for many valuable discussions and critical input on the manuscript. This study was supported by the National Institutes of Health ( GM073732 ) and a seed grant from the Stanford ITI.
PY - 2011/7/1
Y1 - 2011/7/1
N2 - Ribosomes exist as a heterogenous pool of macromolecular complexes composed of ribosomal RNA molecules, ribosomal proteins, and numerous associated "nonribosomal" proteins. To identify nonribosomal proteins that may modulate ribosome activity, we examined the composition of translationally active and inactive ribosomes using a proteomic multidimensional protein identification technology. Notably, the phosphorylated isoform of glycogen synthase, glycogen synthase 1 (GYS1), was preferentially associated with elongating ribosomes. Depletion of GYS1 affected the translation of a subset of cellular mRNAs, some of which encode proteins that modulate protein biosynthesis. These findings argue that GYS1 abundance, by virtue of its ribosomal association, provides a feedback loop between the energy state of the cells and the translation machinery.
AB - Ribosomes exist as a heterogenous pool of macromolecular complexes composed of ribosomal RNA molecules, ribosomal proteins, and numerous associated "nonribosomal" proteins. To identify nonribosomal proteins that may modulate ribosome activity, we examined the composition of translationally active and inactive ribosomes using a proteomic multidimensional protein identification technology. Notably, the phosphorylated isoform of glycogen synthase, glycogen synthase 1 (GYS1), was preferentially associated with elongating ribosomes. Depletion of GYS1 affected the translation of a subset of cellular mRNAs, some of which encode proteins that modulate protein biosynthesis. These findings argue that GYS1 abundance, by virtue of its ribosomal association, provides a feedback loop between the energy state of the cells and the translation machinery.
KW - energy metabolism
KW - mass spectrometry
KW - ribosome filter
KW - translational control
KW - translational profiling
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U2 - 10.1016/j.jmb.2011.04.064
DO - 10.1016/j.jmb.2011.04.064
M3 - Article
C2 - 21570405
AN - SCOPUS:79958706877
SN - 0022-2836
VL - 410
SP - 118
EP - 130
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 1
ER -