@article{78c19c6d5ba74468a718cff993bb221a,
title = "Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria",
abstract = "The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for antimalarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We identify a total of 1879 parasite proteins, and a core set of 1188 proteins quantitatively detected in every biological replicate, providing an extensive picture of the protein repertoire of this stage. This unique data set will allow us to explore key questions about the biology of merosomes and hepatic merozoites.",
keywords = "PEXEL, exoerythrocytic stage, liver stages, malaria, merosomes, proteomics, sporozoites",
author = "Shears, {Melanie J.} and {Sekhar Nirujogi}, Raja and Swearingen, {Kristian E.} and Santosh Renuse and Satish Mishra and {Jaipal Reddy}, Panga and Moritz, {Robert L.} and Akhilesh Pandey and Photini Sinnis",
note = "Funding Information: We thank the Insectary and Parasitology Core Facilities at the Johns Hopkins Malaria Research Institute and in particular Dr. Abhai Tripathi, Dr. Godfree Mlambo, and Chris Kizito for their outstanding work. We are grateful to Bloomberg Philanthropies for supporting these Core Facilities. We thank Dr. Sean Prigge for his assistance with the merosome genome quantitative PCR experiments, and Dr. Scott Lindner for sharing antibodies. We thank the Johns Hopkins School of Medicine Microscopy Facility for assistance with imaging experiments, and Dr. Hoku West-Foyle for his guidance with confocal microscopy and image analysis. This work was supported by a Johns Hopkins Malaria Research Institute Pilot Grant to Drs. Photini Sinnis and Akhilesh Pandey, NIH Grant R01 AI056840 (P.S.), a postdoctoral fellowship to Dr. Melanie Shears from the Johns Hopkins Provost{\textquoteright}s Office and Bloomberg Philanthropies. This work was also funded in part by the National Institutes of Health, grants R01GM087221 and 1S10RR027584 (R.M.), and K25AI119229 (K.S.). Funding for the Zeiss LSM 710 microscope was provided by NIH Grant S10 RR024550. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = sep,
day = "6",
doi = "10.1021/acs.jproteome.9b00324",
language = "English (US)",
volume = "18",
pages = "3404--3418",
journal = "Journal of proteome research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "9",
}