Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation

Ellen Poon; Wendy Keung; Yimin Liang; Rajkumar Ramalingam; Bin Yan; Shaohong Zhang; Anant Chopra; Jennifer Moore; Anthony Herren; Deborah K. Lieu; Hau San Wong; Zhihui Weng; On Tik Wong; Yun Wah Lam; Gordon F. Tomaselli; Christopher Chen; Kenneth R. Boheler; Ronald A. Li

doi:10.1161/CIRCGENETICS.114.000918

Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation

Ellen Poon, Wendy Keung, Yimin Liang, Rajkumar Ramalingam, Bin Yan, Shaohong Zhang, Anant Chopra, Jennifer Moore, Anthony Herren, Deborah K. Lieu, Hau San Wong, Zhihui Weng, On Tik Wong, Yun Wah Lam, Gordon F. Tomaselli, Christopher Chen, Kenneth R. Boheler, Ronald A. Li

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Background - Differentiation of pluripotent human embryonic stem cells (hESCs) to the cardiac lineage represents a potentially unlimited source of ventricular cardiomyocytes (VCMs), but hESC-VCMs are developmentally immature. Previous attempts to profile hESC-VCMs primarily relied on transcriptomic approaches, but the global proteome has not been examined. Furthermore, most hESC-CM studies focus on pathways important for cardiac differentiation, rather than regulatory mechanisms for CM maturation. We hypothesized that gene products and pathways crucial for maturation can be identified by comparing the proteomes of hESCs, hESC-derived VCMs, human fetal and human adult ventricular and atrial CMs. Methods and Results - Using two-dimensional-differential-in-gel electrophoresis, 121 differentially expressed (>1.5-fold; P

Original language	English (US)
Pages (from-to)	427-436
Number of pages	10
Journal	Circulation: Cardiovascular Genetics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1161/CIRCGENETICS.114.000918
State	Published - Jun 11 2015

Keywords

embryonic stem cells
metabolism
PGC1alpha protein
PPARalpha
proteomics
thyroid-stimulating hormone

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine
Genetics(clinical)
Genetics

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10.1161/CIRCGENETICS.114.000918

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Poon, E., Keung, W., Liang, Y., Ramalingam, R., Yan, B., Zhang, S., Chopra, A., Moore, J., Herren, A., Lieu, D. K., Wong, H. S., Weng, Z., Wong, O. T., Lam, Y. W., Tomaselli, G. F., Chen, C., Boheler, K. R., & Li, R. A. (2015). Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation. Circulation: Cardiovascular Genetics, 8(3), 427-436. https://doi.org/10.1161/CIRCGENETICS.114.000918

Poon, E, Keung, W, Liang, Y, Ramalingam, R, Yan, B, Zhang, S, Chopra, A, Moore, J, Herren, A, Lieu, DK, Wong, HS, Weng, Z, Wong, OT, Lam, YW, Tomaselli, GF, Chen, C, Boheler, KR & Li, RA 2015, 'Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation', Circulation: Cardiovascular Genetics, vol. 8, no. 3, pp. 427-436. https://doi.org/10.1161/CIRCGENETICS.114.000918

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abstract = "Background - Differentiation of pluripotent human embryonic stem cells (hESCs) to the cardiac lineage represents a potentially unlimited source of ventricular cardiomyocytes (VCMs), but hESC-VCMs are developmentally immature. Previous attempts to profile hESC-VCMs primarily relied on transcriptomic approaches, but the global proteome has not been examined. Furthermore, most hESC-CM studies focus on pathways important for cardiac differentiation, rather than regulatory mechanisms for CM maturation. We hypothesized that gene products and pathways crucial for maturation can be identified by comparing the proteomes of hESCs, hESC-derived VCMs, human fetal and human adult ventricular and atrial CMs. Methods and Results - Using two-dimensional-differential-in-gel electrophoresis, 121 differentially expressed (>1.5-fold; P",

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AU - Poon, Ellen

AU - Keung, Wendy

AU - Liang, Yimin

AU - Ramalingam, Rajkumar

AU - Yan, Bin

AU - Zhang, Shaohong

AU - Chopra, Anant

AU - Moore, Jennifer

AU - Herren, Anthony

AU - Lieu, Deborah K.

AU - Wong, Hau San

AU - Weng, Zhihui

AU - Wong, On Tik

AU - Lam, Yun Wah

AU - Tomaselli, Gordon F.

AU - Chen, Christopher

AU - Boheler, Kenneth R.

AU - Li, Ronald A.

PY - 2015/6/11

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N2 - Background - Differentiation of pluripotent human embryonic stem cells (hESCs) to the cardiac lineage represents a potentially unlimited source of ventricular cardiomyocytes (VCMs), but hESC-VCMs are developmentally immature. Previous attempts to profile hESC-VCMs primarily relied on transcriptomic approaches, but the global proteome has not been examined. Furthermore, most hESC-CM studies focus on pathways important for cardiac differentiation, rather than regulatory mechanisms for CM maturation. We hypothesized that gene products and pathways crucial for maturation can be identified by comparing the proteomes of hESCs, hESC-derived VCMs, human fetal and human adult ventricular and atrial CMs. Methods and Results - Using two-dimensional-differential-in-gel electrophoresis, 121 differentially expressed (>1.5-fold; P

AB - Background - Differentiation of pluripotent human embryonic stem cells (hESCs) to the cardiac lineage represents a potentially unlimited source of ventricular cardiomyocytes (VCMs), but hESC-VCMs are developmentally immature. Previous attempts to profile hESC-VCMs primarily relied on transcriptomic approaches, but the global proteome has not been examined. Furthermore, most hESC-CM studies focus on pathways important for cardiac differentiation, rather than regulatory mechanisms for CM maturation. We hypothesized that gene products and pathways crucial for maturation can be identified by comparing the proteomes of hESCs, hESC-derived VCMs, human fetal and human adult ventricular and atrial CMs. Methods and Results - Using two-dimensional-differential-in-gel electrophoresis, 121 differentially expressed (>1.5-fold; P

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Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation

Abstract

Keywords

ASJC Scopus subject areas

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