TY - JOUR
T1 - Proteomic analysis of human follicular fluid
T2 - A new perspective towards understanding folliculogenesis
AU - Ambekar, Aditi S.
AU - Nirujogi, Raja Sekhar
AU - Srikanth, Srinivas M.
AU - Chavan, Sandip
AU - Kelkar, Dhanashree S.
AU - Hinduja, Indira
AU - Zaveri, Kusum
AU - Prasad, T. S Keshava
AU - Harsha, H. C.
AU - Pandey, Akhilesh
AU - Mukherjee, Srabani
PY - 2013/7/1
Y1 - 2013/7/1
N2 - Human follicular fluid is a complex body fluid that constitutes the microenvironment of developing follicles in the ovary. Follicular fluid contains a number of proteins that modulate oocyte maturation and ovulation. Information about the protein constituents of follicular fluid may provide a better understanding of ovarian physiology in addition to opening new avenues for investigating ovarian disorders. However, the composition of follicular fluid proteome remains poorly defined. In this study, we carried out SDS-PAGE, OFFGEL and SCX-based separation followed by LC-MS/MS analysis to characterize the proteome of human follicular fluid. We report high confidence identification of 480 proteins, of which 320 have not been described previously in the follicular fluid. The identified proteins belong to diverse functional categories including growth factor and hormones, receptor signaling, enzyme catalysis, defense/immunity and complement activity. Our dataset should serve as a resource for future studies aimed at developing biomarkers for monitoring oocyte and embryo quality, pregnancy outcomes and ovarian disorders. Biological significance: Proteome analysis of human follicular fluid by multi-pronged approach of protein peptide fractionation revealed 480 proteins with high confidence. The identified protein may facilitate the understanding of folliculogenesis. This protein dataset should serve as a useful resource for development of biomarkers for oocyte quality, in vitro fertilization techniques and female infertility.
AB - Human follicular fluid is a complex body fluid that constitutes the microenvironment of developing follicles in the ovary. Follicular fluid contains a number of proteins that modulate oocyte maturation and ovulation. Information about the protein constituents of follicular fluid may provide a better understanding of ovarian physiology in addition to opening new avenues for investigating ovarian disorders. However, the composition of follicular fluid proteome remains poorly defined. In this study, we carried out SDS-PAGE, OFFGEL and SCX-based separation followed by LC-MS/MS analysis to characterize the proteome of human follicular fluid. We report high confidence identification of 480 proteins, of which 320 have not been described previously in the follicular fluid. The identified proteins belong to diverse functional categories including growth factor and hormones, receptor signaling, enzyme catalysis, defense/immunity and complement activity. Our dataset should serve as a resource for future studies aimed at developing biomarkers for monitoring oocyte and embryo quality, pregnancy outcomes and ovarian disorders. Biological significance: Proteome analysis of human follicular fluid by multi-pronged approach of protein peptide fractionation revealed 480 proteins with high confidence. The identified protein may facilitate the understanding of folliculogenesis. This protein dataset should serve as a useful resource for development of biomarkers for oocyte quality, in vitro fertilization techniques and female infertility.
KW - Biomarkers
KW - Folliculogenesis
KW - Fourier transform mass spectrometry
KW - Human follicular fluid
KW - In vitro fertilization
KW - Protein catalog
UR - http://www.scopus.com/inward/record.url?scp=84879396837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879396837&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2013.05.017
DO - 10.1016/j.jprot.2013.05.017
M3 - Article
C2 - 23707233
AN - SCOPUS:84879396837
SN - 1874-3919
VL - 87
SP - 68
EP - 77
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -