Proteomic Alterations Associated with Oral Cancer Patients with Tobacco Using Habits

Firdous Ahmad Bhat, Sonali V. Mohan, Shankargouda Patil, Jayshree Advani, Mohd Younis Bhat, Krishna Patel, Kiran K. Mangalaparthi, Keshava K. Datta, Samapika Routray, Neeta Mohanty, Bipin Nair, S. Ganesh Mandakulutur, Arnab Pal, David Sidransky, Jay Gopal Ray, Harsha Gowda, Aditi Chatterjee

Research output: Contribution to journalArticlepeer-review


Tobacco abuse is a major risk factor associated with the development of oral squamous cell carcinoma. Differences in molecular aberrations induced by tobacco exposure by chewing or smoking form are not well studied in case of oral cancer. We used tandem mass tag-based quantitative proteomic approach to delineate proteomic alterations in oral cancer patients based on their history of tobacco using habits (patients who chewed tobacco, patients who smoked tobacco, and those with no history of tobacco consumption). Our data identified distinct dysregulation of biological processes and pathways in each patient cohort. Bioinformatics analysis of dysregulated proteins identified in our proteomic study revealed dysregulation of collagen formation and antigen processing/presentation pathway in oral cancer patients who smoked tobacco, whereas proteins associated with the process of keratinization showed enrichment in patients who chewed tobacco. In addition, we identified overexpression of proteins involved in immune pathways and downregulation of muscle contraction-mediated signaling events in all three cohorts, irrespective of tobacco using habits. This study lays the groundwork for identification of protein markers that may aid in identification of high-risk patients for cancer development based on the history of tobacco exposure habits.

Original languageEnglish (US)
Pages (from-to)255-268
Number of pages14
JournalOMICS A Journal of Integrative Biology
Issue number4
StatePublished - Apr 2021


  • immune signaling
  • muscle contraction
  • oral cancer
  • proteomics
  • smoking
  • tobacco

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics


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