Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes

Jean Philippe Gagné, Maxim Isabelle, Ken Sin Lo, Sylvie Bourassa, Michael J. Hendzel, Valina Dawson, Ted M Dawson, Guy G. Poirier

Research output: Contribution to journalArticle

Abstract

Poly(ADP-ribose) (pADPr) is a polymer assembled from the enzymatic polymerization of the ADPribosyl moiety of NAD by poly(ADP-ribose) polymerases (PARPs). The dynamic turnover of pADPr within the cell is essential for a number of cellular processes including progression through the cell cycle, DNA repair and the maintenance of genomic integrity, and apoptosis. In spite of the considerable advances in the knowledge of the physiological conditions modulated by poly(ADP-ribosyl)ation reactions, and notwithstanding the fact that pADPr can play a role of mediator in a wide spectrum of biological processes, few pADPr binding proteins have been identified so far. In this study, refined in silico prediction of pADPr binding proteins and large-scale mass spectrometry-based proteome analysis of pADPr binding proteins were used to establish a comprehensive repertoire of pADPr-associated proteins. Visualization and modeling of these pADPr-associated proteins in networks not only reflect the widespread involvement of poly(ADP-ribosyl)ation in several pathways but also identify protein targets that could shed new light on the regulatory functions of pADPr in normal physiological conditions as well as after exposure to genotoxic stimuli.

Original languageEnglish (US)
Pages (from-to)6959-6976
Number of pages18
JournalNucleic Acids Research
Volume36
Issue number22
DOIs
StatePublished - Dec 2008

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Poly Adenosine Diphosphate Ribose
Proteome
Carrier Proteins
Adenosine Diphosphate
Biological Phenomena
poly(ADP)-ribosylated proteins
Proteins
Poly(ADP-ribose) Polymerases
DNA Repair
Polymerization
Computer Simulation
NAD
Mass Spectrometry
Cell Cycle
Polymers
Maintenance
Apoptosis

ASJC Scopus subject areas

  • Genetics

Cite this

Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. / Gagné, Jean Philippe; Isabelle, Maxim; Lo, Ken Sin; Bourassa, Sylvie; Hendzel, Michael J.; Dawson, Valina; Dawson, Ted M; Poirier, Guy G.

In: Nucleic Acids Research, Vol. 36, No. 22, 12.2008, p. 6959-6976.

Research output: Contribution to journalArticle

Gagné, Jean Philippe ; Isabelle, Maxim ; Lo, Ken Sin ; Bourassa, Sylvie ; Hendzel, Michael J. ; Dawson, Valina ; Dawson, Ted M ; Poirier, Guy G. / Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. In: Nucleic Acids Research. 2008 ; Vol. 36, No. 22. pp. 6959-6976.
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