TY - JOUR
T1 - Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine
AU - Oliveira, C. J.
AU - Anatriello, E.
AU - de Miranda-Santos, I. K.
AU - Francischetti, I. M.
AU - Sá-Nunes, A.
AU - Ferreira, B. R.
AU - Ribeiro, J. M.C.
N1 - Funding Information:
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP through grant 2009/53645-3 to IKMS, and scholarships to EA (2007/50869-2) and CJFO (2006/54985-4); by the Brazilian National Science Foundation-CNPq through grants 559603/2009-6 and 471946/2010-9 to IKMS, Fundação de Amparo à Pesquisa do Estado de Minas Gerais- FAPEMIG and the Intramural Research Program of the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health. We thank the Research Technology Branch under Dr. Robert Hohman for preparing the gel and mass spectrometric data collection and analysis, and to the NIAID intramural editor Brenda Rae Marshall for assistance.
PY - 2013/12
Y1 - 2013/12
N2 - One dimensional gel electrophoresis was used to separate proteins from the saliva of Rhipicephalus sanguineus female ticks fed on rabbits. Gel slices were subjected to tryptic digestion and analyzed by reversed-phase HPLC followed by MS/MS analysis. The data were compared to a database of salivary proteins of the same tick and to the predicted proteins of the host. Saliva was obtained by either pilocarpine or dopamine stimulation of partially fed ticks. Electrophoretic separations of both yielded products that were identified by mass spectrometry, although the pilocarpine-derived sample was of much better quality. The majority of identified proteins were of rabbit origin, indicating the recycling of the host proteins in the tick saliva, including hemoglobin, albumin, haptoglobin, transferring, and a plasma serpin. The few proteins found that were previously associated with parasitism and blood feeding include 2 glycine-rich, cement-like proteins, 2 lipocalins, and a thyropin protease inhibitor. Among other of the 19 tick proteins identified, albeit with undefined roles, were SPARC and cyclophilin A. This catalog provides a resource that can be mined for secreted molecules that play a role in tick-host interactions.
AB - One dimensional gel electrophoresis was used to separate proteins from the saliva of Rhipicephalus sanguineus female ticks fed on rabbits. Gel slices were subjected to tryptic digestion and analyzed by reversed-phase HPLC followed by MS/MS analysis. The data were compared to a database of salivary proteins of the same tick and to the predicted proteins of the host. Saliva was obtained by either pilocarpine or dopamine stimulation of partially fed ticks. Electrophoretic separations of both yielded products that were identified by mass spectrometry, although the pilocarpine-derived sample was of much better quality. The majority of identified proteins were of rabbit origin, indicating the recycling of the host proteins in the tick saliva, including hemoglobin, albumin, haptoglobin, transferring, and a plasma serpin. The few proteins found that were previously associated with parasitism and blood feeding include 2 glycine-rich, cement-like proteins, 2 lipocalins, and a thyropin protease inhibitor. Among other of the 19 tick proteins identified, albeit with undefined roles, were SPARC and cyclophilin A. This catalog provides a resource that can be mined for secreted molecules that play a role in tick-host interactions.
KW - Dopamine
KW - Pilocarpine
KW - Proteome
KW - Rhipicephalus sanguineus
KW - Tick saliva
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U2 - 10.1016/j.ttbdis.2013.05.001
DO - 10.1016/j.ttbdis.2013.05.001
M3 - Article
C2 - 24029695
AN - SCOPUS:84887535380
VL - 4
SP - 469
EP - 477
JO - Ticks and Tick-borne Diseases
JF - Ticks and Tick-borne Diseases
SN - 1877-959X
IS - 6
ER -