Proteome of murine jejunal brush border membrane vesicles

Mark Donowitz, Siddharth Singh, Farah F. Salahuddin, Boris M. Hogema, Yueping Chen, Marjan Gucek, Robert N. Cole, Nicholas C. Zachos, Olga Kovbasnjuk, Lynne A. Lapierre, Nellie Broere, James Goldenring, Hugo Dejonge, Xuhang Li

Research output: Contribution to journalArticle

Abstract

The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg2+) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na+I- symporter (NIS), and non-gastric H +/K+-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.

Original languageEnglish (US)
Pages (from-to)4068-4079
Number of pages12
JournalJournal of proteome research
Volume6
Issue number10
DOIs
StatePublished - Oct 2007

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Keywords

  • Brush border
  • LC-MS/MS
  • Murine small intestine
  • Proteome
  • Transport protein

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Donowitz, M., Singh, S., Salahuddin, F. F., Hogema, B. M., Chen, Y., Gucek, M., Cole, R. N., Zachos, N. C., Kovbasnjuk, O., Lapierre, L. A., Broere, N., Goldenring, J., Dejonge, H., & Li, X. (2007). Proteome of murine jejunal brush border membrane vesicles. Journal of proteome research, 6(10), 4068-4079. https://doi.org/10.1021/pr0701761