Proteolytic degradation of protamine during thiol‐induced nuclear decondensation in rabbit spermatozoa

T. S.K. Chang, Barry R. Zirkin

Research output: Contribution to journalArticle

Abstract

Rabbit sperm nuclei decondensed when incubated with dithiothreitol and Triton X‐100. Protamine isolated from these nuclei during the course of treatment exhibited marked and progressive degradation. Timed interval experiments revealed that the degradation of protamine significantly preceded sperm nuclear decondensation and reached its maximum extent before decondensation was completed. Studies with protease inhibitors demonstrated that proteolysis was involved in both nuclear decondensation and protamine degradation.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalJournal of Experimental Zoology
Volume204
Issue number2
DOIs
StatePublished - May 1978

ASJC Scopus subject areas

  • Animal Science and Zoology

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