Protein tyrosine-O-sulfation in bovine ocular tissues

Yogita Kanan, Robert A. Hamilton, Kevin L. Moore, Muayyad R. Al-Ubaidi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations


Protein tyrosine-O-sulfation (aka sulfonation, sulfation) has been recently identified as an important posttranslational modification for vision. In the absence of sulfation, rod and cone ERGs are reduced and vision is compromised. With the goal of identifying the sulfated retinal proteins in the eye, various bovine ocular tissues were examined. To that end, the cornea, aqueous humor, lens, iris, vitreous humor, retina, RPE, and sclera were examined for the presence of sulfated proteins using the PSG2 antibody, which has previously been used to identify and isolate sulfated proteins from various tissues. Data presented show that the vitreous humor is the richest source of sulfated proteins in the eye while the lens expressed the least. Furthermore, the sulfated proteins in the retinal pigment epithelium and retina were either soluble members of the extracellular matrix and/or membrane-associated proteins. Future plans are directed towards the identification of the different sulfated proteins by immunoaffinity purification with the PSG2 antibody and the determination of the role of sulfation in the function of these ocular proteins.

Original languageEnglish (US)
Title of host publicationRetinal Degenerative Diseases
EditorsMatthew LaVail, Robert Anderson, Christian Grimm, John Ash, Joe Hollyfield
Number of pages7
StatePublished - 2012
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598


  • Insoluble Interphotoreceptor Matrix
  • Insoluble Interretinal Pigment Epithelium Matrix
  • Protein Tyrosine-O-Sulfation
  • Soluble Interphotoreceptor Matrix
  • Soluble Interretinal Pigment Epithelium Matrix
  • Sulfation
  • Tyrosylprotein Sulfotransferase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Protein tyrosine-O-sulfation in bovine ocular tissues'. Together they form a unique fingerprint.

Cite this