TY - JOUR
T1 - Protein traffic between distinct plasma membrane domains
T2 - Isolation and characterization of vesicular carriers involved in transcytosis
AU - Sztul, Elizabeth
AU - Kaplin, Adam
AU - Saucan, Lucian
AU - Palade, George
N1 - Funding Information:
We wish to thank Dr. J.-P Kraehenbuhl for providing us with the myeloma cell line producing anti-pIgA-R antibodies. We are grateful to Drs. Ann Hubbard, David Bole, Robert Levenson, and John Woods for their kind gifts of antibodies. We thank Kathryn Howell for her advice during the course of this work. This research was supported by a grant from the National Institutes of Health (GM 27303 to G. E. P) and by a pilot project from the Yale University School of Medicine, Liver Center Dk 34969 (to E. S. S.).
PY - 1991/1/11
Y1 - 1991/1/11
N2 - We have Isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi elements, basolateral plasma membrane, as well as early endosomal components. The fraction was analyzed by biochemical and Immunological procedures. Antibodies raised against the proteins in the fraction recognized a single 108K antigen. Based on its subcellular distribution, the 108K antigen may represent a novel marker for transcytotic vesicular carriers.
AB - We have Isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi elements, basolateral plasma membrane, as well as early endosomal components. The fraction was analyzed by biochemical and Immunological procedures. Antibodies raised against the proteins in the fraction recognized a single 108K antigen. Based on its subcellular distribution, the 108K antigen may represent a novel marker for transcytotic vesicular carriers.
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U2 - 10.1016/0092-8674(91)90210-P
DO - 10.1016/0092-8674(91)90210-P
M3 - Article
C2 - 1986870
AN - SCOPUS:0026099060
SN - 0092-8674
VL - 64
SP - 81
EP - 89
JO - Cell
JF - Cell
IS - 1
ER -