Protein traffic between distinct plasma membrane domains: Isolation and characterization of vesicular carriers involved in transcytosis

Elizabeth Sztul, Adam Kaplin, Lucian Saucan, George Palade

Research output: Contribution to journalArticle

Abstract

We have Isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi elements, basolateral plasma membrane, as well as early endosomal components. The fraction was analyzed by biochemical and Immunological procedures. Antibodies raised against the proteins in the fraction recognized a single 108K antigen. Based on its subcellular distribution, the 108K antigen may represent a novel marker for transcytotic vesicular carriers.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalCell
Volume64
Issue number1
DOIs
StatePublished - Jan 11 1991

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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