@article{777bed38b505495887ec0d1a40b8a993,
title = "Protein topology of presenilin 1",
abstract = "Mutations in a gene encoding a multitransmembrane protein, termed presenilin 1 (PS1), are causative in the majority of early-onset cases of AD. To determine the topology of PS1, we utilized two strategies: first, we tested whether putative transmembranes are sufficient to export a protease- sensitive substrate across a lipid bilayer; and second, we examined the binding of antibodies to specific PSi epitopes in cultured cells selectively permeabilized with the pore-forming toxin, streptolysin-O. We document that the 'loop,' N-terminal, and C-terminal domains of PS1 are oriented toward the cytoplasm.",
author = "Andrew Doan and Gopal Thinakaran and Borchelt, {David R.} and Slunt, {Hilda H.} and Tamara Ratovitsky and Marcia Podlisny and Selkoe, {Dennis J.} and Mary Seeger and Gandy, {Samuel E.} and Price, {Donald L.} and Sisodia, {Sangram S.}",
note = "Funding Information: We thank Dr. Carolyn Machamer for her insightful discussions on the SLO experiments and Ms. Frances Davenport for her excellent technical assistance with the cell culture. This work was supported by the U. S. Public Health Service, National Institute of Health grants NIH AG05146, NS 20471; AG11508, AG09464, and AG10491 to S. E. G.; and AG05689 to M. S.; and by a training grant from the Medical Scientist Training Program (A. D.); and by grants from the Adler Foundation, the Develbiss Fund, and the Alzheimers Association (D. R. B. and S. S. S.); D. L. P. is the recipient of a Javitz Neuroscience Investigator Award (NIH NS10580); D. L. P. and D. R. B. are the recipients of a Leadership and Excellence in Alzheimer's Disease (LEAD) Award (NIH AG07914); S. S. S. is the recipient of an Alzheimers Association Zenith Award. ",
year = "1996",
month = nov,
doi = "10.1016/S0896-6273(00)80232-9",
language = "English (US)",
volume = "17",
pages = "1023--1030",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",
}