Protein sulfhydration

Research output: Contribution to journalArticle

Abstract

Hydrogen sulfide (H2S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H2S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.

Original languageEnglish (US)
Pages (from-to)79-90
Number of pages12
JournalMethods in Enzymology
Volume555
DOIs
StatePublished - 2015

Fingerprint

Cysteine
Gasotransmitters
Neurodegenerative diseases
Biological Phenomena
Hydrogen Sulfide
Post Translational Protein Processing
Biotin
Neurodegenerative Diseases
Parkinson Disease
Heart Diseases
Assays
Proteins
Switches
persulfides

Keywords

  • Hydrogen sulfide
  • Maleimide assay
  • Modified biotin switch
  • Nitrosylation
  • Sulfhydration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Protein sulfhydration. / Paul, Bindu; Snyder, Solomon H.

In: Methods in Enzymology, Vol. 555, 2015, p. 79-90.

Research output: Contribution to journalArticle

@article{8625c0c1eb544f4b8b2253df73dbae5f,
title = "Protein sulfhydration",
abstract = "Hydrogen sulfide (H2S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H2S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.",
keywords = "Hydrogen sulfide, Maleimide assay, Modified biotin switch, Nitrosylation, Sulfhydration",
author = "Bindu Paul and Snyder, {Solomon H}",
year = "2015",
doi = "10.1016/bs.mie.2014.11.021",
language = "English (US)",
volume = "555",
pages = "79--90",
journal = "ImmunoMethods",
issn = "1046-2023",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Protein sulfhydration

AU - Paul, Bindu

AU - Snyder, Solomon H

PY - 2015

Y1 - 2015

N2 - Hydrogen sulfide (H2S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H2S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.

AB - Hydrogen sulfide (H2S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H2S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.

KW - Hydrogen sulfide

KW - Maleimide assay

KW - Modified biotin switch

KW - Nitrosylation

KW - Sulfhydration

UR - http://www.scopus.com/inward/record.url?scp=84924285607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924285607&partnerID=8YFLogxK

U2 - 10.1016/bs.mie.2014.11.021

DO - 10.1016/bs.mie.2014.11.021

M3 - Article

C2 - 25747476

AN - SCOPUS:84924285607

VL - 555

SP - 79

EP - 90

JO - ImmunoMethods

JF - ImmunoMethods

SN - 1046-2023

ER -