Abstract
Treatment of purified simian virus 40 large T antigen (LT) with protein phosphatase 2A stimulates LT-dependent DNA unwinding and replication (D. M. Virshup, M. G. Kauffman, and T. J. Kelly, EMBO J. 8: 3891-3898, 1989). The specificity of the catalytic subunit of protein phosphatase 2A toward LT was investigated by two-dimensional peptide mapping. Increasing amounts of phosphatase sequentially removed the phosphates from serine residues 120, 123, 677, and perhaps 679, residues which have been implicated in regulating the DNA-binding activity of LT.
Original language | English (US) |
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Pages (from-to) | 2098-2101 |
Number of pages | 4 |
Journal | Journal of virology |
Volume | 65 |
Issue number | 4 |
State | Published - 1991 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology