Protein O-GlcNAcylation in diabetes and diabetic complications

Junfeng M.a., Gerald Warren Hart

Research output: Contribution to journalArticle

Abstract

The post-translational modification of serine and threonine residues of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is highly ubiquitous, dynamic and inducible. Protein O-GlcNAcylation serves as a key regulator of critical biological processes including transcription, translation, proteasomal degradation, signal transduction and apoptosis. Increased O-GlcNAcylation is directly linked to insulin resistance and to hyperglycemia-induced glucose toxicity, two hallmarks of diabetes and diabetic complications. In this review, we briefly summarize what is known about protein O-GlcNAcylation and nutrient metabolism, as well as discuss the commonly used tools to probe changes of O-GlcNAcylation in cultured cells and in animal models. We then focus on some key proteins modified by O-GlcNAc, which play crucial roles in the etiology and progression of diabetes and diabetic complications. Proteomic approaches are also highlighted to provide a system view of protein O-GlcNAcylation. Finally, we discuss how aberrant O-GlcNAcylation on certain proteins may be exploited to develop methods for the early diagnosis of pre-diabetes and/or diabetes.

Original languageEnglish (US)
Pages (from-to)365-380
Number of pages16
JournalExpert Review of Proteomics
Volume10
Issue number4
DOIs
StatePublished - Aug 2013

Fingerprint

Diabetes Complications
Medical problems
Proteins
Biological Phenomena
Signal transduction
Acetylglucosamine
Threonine
Transcription
Post Translational Protein Processing
Metabolism
Hyperglycemia
Proteomics
Serine
Nutrients
Toxicity
Insulin Resistance
Early Diagnosis
Cultured Cells
Signal Transduction
Animals

Keywords

  • diabetes
  • diabetic complications
  • hyperglycemia
  • insulin resistance
  • O-GlcNAc
  • O-GlcNAcomics
  • proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Protein O-GlcNAcylation in diabetes and diabetic complications. / M.a., Junfeng; Hart, Gerald Warren.

In: Expert Review of Proteomics, Vol. 10, No. 4, 08.2013, p. 365-380.

Research output: Contribution to journalArticle

M.a., Junfeng ; Hart, Gerald Warren. / Protein O-GlcNAcylation in diabetes and diabetic complications. In: Expert Review of Proteomics. 2013 ; Vol. 10, No. 4. pp. 365-380.
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