Protein nitrogen and DNA accumulation in early chick embryos cultured on several purified yolk proteins1-3

James D. Yager, Norman W. Klein

Research output: Contribution to journalArticlepeer-review

Abstract

Chick embryos containing 11-13 pairs of somites were cultured on media containing either yolk fractions or purified egg yolk proteins in order to determine their ability to support growth (protein nitrogen and DNA accumulation). The heterogeneous high density fraction which contains lipovitellin, livetin, and phosvitin supported levels of explant growth that were comparable to the high levels obtained on a medium containing fresh yolk plus white. No single purified yolk protein was capable of supporting high levels of explant growth. However, when the yolk lipoprotein, lipovitellin was combined in a single medium with specific components of the livetin fraction, high levels of growth were achieved. Neither phosvitin nor the low density fraction of egg yolk appeared to contribute to embryo growth at the early stage of development studied.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalDevelopmental biology
Volume39
Issue number1
DOIs
StatePublished - Jul 1974
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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