Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions

Samuel Herianto, Chien Sheng Chen, Heng Zhu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Interactions between specific lipids and proteins can provide important information regarding the functions of proteins and lipids. One of the novel and powerful methods to identify lipid–protein interactions is using protein microarrays and liposomes. Liposomes are spherical vesicles that are surrounded by phospholipid bilayers in which the lipid of interest can be incorporated. Thus, liposomes can be used to detect lipid–protein interactions and to analyze interactions between thousands of proteins and a small number of lipids with a single experiment. This chapter presents the methods and procedures for using protein microarray assays and liposome fabrication to analyze protein–lipid interactions. Up-to-date research reports are also reviewed briefly.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages191-199
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2003
ISSN (Print)1064-3745

Keywords

  • High throughput
  • Liposomes
  • Protein microarrays
  • Protein–lipid interactions

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Herianto, S., Chen, C. S., & Zhu, H. (2019). Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions. In Methods in Molecular Biology (pp. 191-199). (Methods in Molecular Biology; Vol. 2003). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9512-7_10