Protein metabolism and fibrosis in experimental canine obstructive lymphedema

Kenneth R. Knight, Peter A. Collopy, John J. McCann, Craig Vander Kolk, Serena A. Coe, Ronald M. Barton, Hung Chi Chen, Bernard Mc C. O'Brien

Research output: Contribution to journalArticle

Abstract

Obstructive lymphedema is a pathologic condition resulting in the accumulation and stagnation of serum proteins in the lymphatics and interstitial spaces. In a canine model of obstructive lymphedema, one limb was rendered lymphedematous, and various biochemical parameters were determined in this and an unaffected control limb. Both lymph and interstitial fluid had significantly decreased acid proteinase activity (comprising mostly cathepsin D-like enzymes) and neutral proteinase activity (comprising metallo, sulfhydryl, and serine proteinases, and collagenase). Possible reasons for these decreases could be: (1) saturation of macrophages and their surrounding environment with whole or partially digested proteins, or (2) elevation in the levels of circulating inhibitors like α2-macroglobulin. The lymphedematous skin was significantly thicker than control skin and had elevated levels of collagen. However, unlike some fibrotic conditions, the relative proportions of types I, III, and V collagen, as determined by pepsin solubilization and neutral salt fractionation of the collagen fibrils, were similar to those found in normal skin. It is speculated that a decrease in the breakdown of collagen by collagenase and a continuing synthesis of collagen by fibroblasts led to an imbalance in favor of collagen deposition in the skin.

Original languageEnglish (US)
Pages (from-to)558-566
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume110
Issue number5
StatePublished - Jan 1 1987
Externally publishedYes

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Lymphedema
Metabolism
Canidae
Fibrosis
Collagen
Skin
Collagenases
Proteins
4 alpha-glucanotransferase
Peptide Hydrolases
Extremities
Collagen Type V
Macroglobulins
Cathepsin D
Collagen Type III
Pepsin A
Extracellular Fluid
Serine Proteases
Lymph
Collagen Type I

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Knight, K. R., Collopy, P. A., McCann, J. J., Vander Kolk, C., Coe, S. A., Barton, R. M., ... O'Brien, B. M. C. (1987). Protein metabolism and fibrosis in experimental canine obstructive lymphedema. The Journal of Laboratory and Clinical Medicine, 110(5), 558-566.

Protein metabolism and fibrosis in experimental canine obstructive lymphedema. / Knight, Kenneth R.; Collopy, Peter A.; McCann, John J.; Vander Kolk, Craig; Coe, Serena A.; Barton, Ronald M.; Chen, Hung Chi; O'Brien, Bernard Mc C.

In: The Journal of Laboratory and Clinical Medicine, Vol. 110, No. 5, 01.01.1987, p. 558-566.

Research output: Contribution to journalArticle

Knight, KR, Collopy, PA, McCann, JJ, Vander Kolk, C, Coe, SA, Barton, RM, Chen, HC & O'Brien, BMC 1987, 'Protein metabolism and fibrosis in experimental canine obstructive lymphedema', The Journal of Laboratory and Clinical Medicine, vol. 110, no. 5, pp. 558-566.
Knight KR, Collopy PA, McCann JJ, Vander Kolk C, Coe SA, Barton RM et al. Protein metabolism and fibrosis in experimental canine obstructive lymphedema. The Journal of Laboratory and Clinical Medicine. 1987 Jan 1;110(5):558-566.
Knight, Kenneth R. ; Collopy, Peter A. ; McCann, John J. ; Vander Kolk, Craig ; Coe, Serena A. ; Barton, Ronald M. ; Chen, Hung Chi ; O'Brien, Bernard Mc C. / Protein metabolism and fibrosis in experimental canine obstructive lymphedema. In: The Journal of Laboratory and Clinical Medicine. 1987 ; Vol. 110, No. 5. pp. 558-566.
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