Protein kinase CK2 phosphorylates the Nef protein from a neurovirulent simian immunodeficiency virus

Matthew J. Caples; Janice E. Clements; Sheila A. Barber

doi:10.1016/j.virol.2005.12.028

Protein kinase CK2 phosphorylates the Nef protein from a neurovirulent simian immunodeficiency virus

Matthew J. Caples, Janice E. Clements, Sheila A. Barber

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The Nef protein of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV) is a pluripotent accessory protein that plays a critical role in disease progression. One analogous characteristic of Nef proteins from SIV and HIV is the ability to associate with cellular kinases. We have previously reported that the Nef protein from a macrophage-tropic neurovirulent SIV clone, SIV/17E-Fr, is associated with an unknown kinase activity that is distinct from the p21-associated kinase that interacts with SIVmac239 Nef. Using site-directed mutagenesis and kinase-specific inhibitors, we have identified this kinase as the ubiquitous serine/threonine kinase, protein kinase CK2.

Original language	English (US)
Pages (from-to)	156-164
Number of pages	9
Journal	Virology
Volume	348
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2005.12.028
State	Published - Apr 25 2006

Keywords

Nef
Neurovirulent
Protein kinase CK2
SIV

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2005.12.028

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title = "Protein kinase CK2 phosphorylates the Nef protein from a neurovirulent simian immunodeficiency virus",

abstract = "The Nef protein of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV) is a pluripotent accessory protein that plays a critical role in disease progression. One analogous characteristic of Nef proteins from SIV and HIV is the ability to associate with cellular kinases. We have previously reported that the Nef protein from a macrophage-tropic neurovirulent SIV clone, SIV/17E-Fr, is associated with an unknown kinase activity that is distinct from the p21-associated kinase that interacts with SIVmac239 Nef. Using site-directed mutagenesis and kinase-specific inhibitors, we have identified this kinase as the ubiquitous serine/threonine kinase, protein kinase CK2.",

keywords = "Nef, Neurovirulent, Protein kinase CK2, SIV",

author = "Caples, {Matthew J.} and Clements, {Janice E.} and Barber, {Sheila A.}",

note = "Funding Information: The authors thank Dr. Lorenzo A. Pinna and Dr. Flavio Meggio for generously providing TBB. The authors would also like to thank Lucio Gama and Brandon T. Bullock for their contributions to this work as well as the rest of the Retrovirus Laboratory for helpful discussions. The work was supported by NIH grants NS-35751, MH-070306, and NS-07392.",

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AU - Caples, Matthew J.

AU - Clements, Janice E.

AU - Barber, Sheila A.

N1 - Funding Information: The authors thank Dr. Lorenzo A. Pinna and Dr. Flavio Meggio for generously providing TBB. The authors would also like to thank Lucio Gama and Brandon T. Bullock for their contributions to this work as well as the rest of the Retrovirus Laboratory for helpful discussions. The work was supported by NIH grants NS-35751, MH-070306, and NS-07392.

PY - 2006/4/25

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N2 - The Nef protein of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV) is a pluripotent accessory protein that plays a critical role in disease progression. One analogous characteristic of Nef proteins from SIV and HIV is the ability to associate with cellular kinases. We have previously reported that the Nef protein from a macrophage-tropic neurovirulent SIV clone, SIV/17E-Fr, is associated with an unknown kinase activity that is distinct from the p21-associated kinase that interacts with SIVmac239 Nef. Using site-directed mutagenesis and kinase-specific inhibitors, we have identified this kinase as the ubiquitous serine/threonine kinase, protein kinase CK2.

AB - The Nef protein of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV) is a pluripotent accessory protein that plays a critical role in disease progression. One analogous characteristic of Nef proteins from SIV and HIV is the ability to associate with cellular kinases. We have previously reported that the Nef protein from a macrophage-tropic neurovirulent SIV clone, SIV/17E-Fr, is associated with an unknown kinase activity that is distinct from the p21-associated kinase that interacts with SIVmac239 Nef. Using site-directed mutagenesis and kinase-specific inhibitors, we have identified this kinase as the ubiquitous serine/threonine kinase, protein kinase CK2.

KW - Nef

KW - Neurovirulent

KW - Protein kinase CK2

KW - SIV

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JF - Virology

SN - 0042-6822

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Protein kinase CK2 phosphorylates the Nef protein from a neurovirulent simian immunodeficiency virus

Abstract

Keywords

ASJC Scopus subject areas

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